Library Prep Kits
NEXTFLEX NGS library prep kits for Illumina sequencing platforms
NGS library preparation is a critical process that involves converting raw DNA or RNA samples into a format suitable for sequencing on high-throughput platforms. This preparation includes fragmenting the nucleic acids, repairing ends, adding sequencing adapters, and enriching target regions—all essential steps to ensure accurate and comprehensive sequencing data. Our NEXTFLEX® kits simplify these steps with optimized protocols and high-quality reagents, making the library preparation process more efficient and reliable. Furthermore, our kits are designed for full compatibility with leading sequencing platforms such as Illumina® and Element Biosciences™, ensuring they integrate smoothly into your existing laboratory setups and workflows.
We offer a comprehensive portfolio of NEXTFLEX® Next-Generation Sequencing (NGS) library preparation kits designed to streamline your genomic workflows and deliver exceptional results. Recognizing the demands of modern research, we've ensured that our products enable seamless integration with automated liquid handling systems. This focus on automation allows for increased throughput, reduced hands-on time, and enhanced reproducibility, empowering researchers to accelerate their discoveries while maintaining robustness from the very beginning of their workflows.
We provide a diverse range of library prep kits tailored to various applications in genomics research. For DNA sequencing, our kits support whole-genome sequencing , targeted sequencing, and amplicon sequencing, facilitating the exploration of genetic variations and structural genomic changes. In transcriptomics, our RNA-seq kits are designed for mRNA sequencing, total RNA sequencing, and strand-specific RNA sequencing, enabling detailed insights into gene expression and regulation. We also offer specialized kits for small RNA and microRNA sequencing, which are vital for studying gene silencing and post-transcriptional regulation mechanisms. Complementing our core kits, we provide an extensive selection of accessory products —including Universal Blockers, NGS Cleanup Beads, ribodepletion reagents , and barcodes—that are compatible with most other library prep kits on the market including Illumina™, NEB™, Watchmaker™, Twist™, etc. This versatility allows researchers to enhance their existing workflows without the need to alter established protocols, offering cost-effective solutions that maintain flexibility and performance.
- NGS adapters: Barcoded adapters for all of your NGS multiplexing needs.
- Small RNA-seq library prep: Gene silencing and post-transcriptional regulation of gene expression can be investigated by small RNA-seq, which allows for the profiling of all of the small RNA and miRNA present in samples.
- RNA-seq library prep: Profiling for the analysis of the expression level of RNAs in a given cell or population of cells.
- DNA-seq library prep: Library prep kits for whole genome sequencing.
- Targeted sequencing: Targeted resequencing for a highly targeted approach that analyses specific genomic regions.
- 16S rRNA sequencing: 16S amplicon panels used to identify and compare bacteria present within an environmental sample.
- Single cell DNA-sequencing: Amplification of DNA from single cells and other limited DNA templates for NGS and other applications.
- Reproductive genetic research: Streamlined PGT-A preparation
For research use only. Not for use in diagnostic procedures.
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The NEXTFLEX® NGS Cleanup Beads are Solid-phase reversible immobilization (SPRI) beads that efficiently purify nucleic acids using reversible magnetic bead binding. They are ideal for PCR and NGS cleanup, removing unwanted components like soluble contaminants, nucleotides, primers, salts, and adapters. They selectively bind DNA by size with high recovery and consistent results. These SPRI beads can be used manually or in any automated liquid handler, depending on sample throughput.
The NEXTFLEX™ HT Agrigenomics Low-pass WGS kit is designed for the highly efficient conversion of DNA, from both high- and low-quality samples from breeders, into Illumina® and Element® compatible libraries and can be paired with analysis solutions from CURIO. Curio Genomics is a bioinformatics software company focused on accelerating agriculture genomics research.
The NEXTFLEX® Rapid XP V2 DNA-seq kit for Metagenomics offers a simplified DNA-seq library preparation workflow for whole genome sequencing (WGS) of microbial communities on Illumina® and Element Biosciences® platforms. Libraries produced using this kit are normalized during library construction using proprietary NEXTFLEX normalization beads, delivering consistent mass and sequencing cluster density for all samples in a library pool. The NEXTFLEX Rapid XP V2 DNA-seq kit for Metagenomics is now bundled with the CosmosID-HUB analysis solution, delivering user-friendly access to version-controlled pipelines for whole genome sequencing.
The NEXTFLEX™ UDI-UMI Barcodes consist of 96 unique dual-index (UDI) barcodes, each with a 10-base pair UDI and a 9-base pair unique molecular identifier (UMI). These UMI adapters are designed for multiplexing both DNA and RNA NGS libraries on all Illumina® and Element® sequencers and are compatible with any TruSeq® style library prep kit.
The DepleteX® Mitochondrial DNA Depletion kit employs a straightforward CRISPR-based method to selectively remove unwanted human molecules derived from mitochondrial nucleic acid before sequencing. Researchers can achieve more informative results while sequencing less in samples with abundant mitochondrial content such as ATAC-seq NGS libraries.
Elevate your RNA-seq library quality by targeting and removing excessive rRNA from human, mouse, or rat samples. This advanced protocol is engineered for high-throughput screening, shifting the ribodepletion process to post-library preparation and facilitating efficient processing of up to 96 multiplexed libraries simultaneously.
Revolutionize your RNA-seq analysis by purging excessive rRNA from human, mouse, rat, and bacterial samples. Our cutting-edge protocol is crafted for high-throughput applications by enabling depletion on multiplexed pools of samples.
The DepleteX® Single Cell RNA Boost Kit employs a CRISPR-based method to selectively remove unwanted reads allowing you to cut through the noise with minimal impact on your workflow and maximum confidence in your results.
The CRISPRclean® Stranded Total RNA Prep with rRNA Depletion (Human, Mouse, Rat) is a total RNA library prep leveraging a CRISPR-powered depletion workflow facilitating the detection of lower expressing and biologically relevant transcripts from complex samples.
Now you can enhance detection of low-expressing transcripts using the Jumpcode Genomics comprehensive RNA depletion panel. The DepleteX® High Expressing RNA Depletion kit selectively removes the most highly expressed transcripts in vitro before sequencing. This process reallocates approximately 70% of sequencing reads to unique, biologically relevant transcripts, enabling enhanced detection of low-expressing transcripts and rare isoforms.
The DepleteX® Globin Depletion Kit selectively removes highly expressed globin transcripts (HBA1, HBA2, HBB, & HBD) in vitro before sequencing. This process redirects sequencing resources toward unique biologically relevant transcripts, maximizing UMI and gene sensitivity.
The DepleteX® Nasopharyngeal Microbial RNA Boost Kit selectively removes highly expressed transcripts in vivo before sequencing. This process redistributes sequencing clusters toward microbial content and pathogens, enabling maximum discovery.
Jumpcode Genomics’ CRISPRclean™ Plus Stranded Total RNA Prep with rRNA Depletion (Human, Mouse, Rat, Pan Bacteria) kit is a directional RNA library prep leveraging a CRISPR-powered depletion workflow facilitating the detection of lower expressing and biologically relevant transcripts from complex samples. An optimized workflow with 9-hour total assay time and 3.5 hours of hands-on time (HOT) is made up of 7 simple steps with multiple safe stopping points.
NEXTFLEX Neo NGS RUO kits offer robust genome coverage to identify variants in over 390 genes.
The NEXTFLEX® blood miRNA blockers are an oligonucleotide mix which reduce the abundance of specific microRNA abundant in total RNA isolated from human whole blood and plasma in small RNA libraries. They are intended to be used with the NEXTFLEX® Small RNA-Seq Kit v4.
Library preparation kits for 16S V1-V3 rRNA bacterial metagenomics analysis for Illumina® and Element Biosciences® NGS platforms.
The NEXTFLEX® Rapid XP V2 DNA kit offers a simplified DNA library preparation workflow for sequencing on Illumina® and Element Biosciences® platforms. Libraries produced using this kit are normalized during library construction using proprietary NEXTFLEX® normalization beads, delivering consistent mass and sequencing cluster density for all samples in a library pool.
The DOPlify® WGA V2 kit performs fast whole genome amplification on single cells or limited template DNA samples, allowing for the cell chromosome copy number status to be determined.
The NEXTFLEX® RiboNaut™ rRNA depletion kit (human / mouse / rat) is an effective method to remove rRNA contamination from mammalian total RNA while enabling labs to interrogate additional RNA species in a sample, not only limited to intact mRNAs.
The NEXTFLEX® Cell Free DNA-Seq Kit 2.0 can produce libraries from 10 ng of cell free DNA for circulating tumor DNA (ctDNA) or cell free fetal DNA (cffDNA) analysis, for sequencing on Illumina® or Element Biosciences® NGS platforms in two hours.
The NEXTFLEX® Unique Dual Index Barcodes are barcoded adapters that can be used with single read or paired-end read sequencing, and are compatible with NEXTFLEX library prep kits designed for sequencing on Illumina® and Element® platforms. They are compatible with any library prep workflows that involve the ligation of adapters to adenylated fragments (TruSeq® style).
The NEXTFLEX™ Rapid Directional RNA-seq library prep kit 2.0 produces libraries for Illumina® and Element Biosciences® sequencing instruments with high coverage uniformity, low duplication rates, strand specificity and minimal rRNA contamination when used with the NEXTFLEX Poly(A) Beads 2.0 (10 ng – 5 μg) or NEXTFLEX RiboNaut™ rRNA depletion kit (human, mouse, rat) (5 ng – 1 μg).
The NEXTFLEX 16S V3 – V4 Amplicon-Seq Library Prep Kit is designed for the preparation of multiplexed amplicon libraries that span the hypervariable domains three through four (V3 and V4) of microbial 16S ribosomal RNA (rRNA) genes. These libraries are compatible with paired-end sequencing on the Illumina® and Element Biosciences® sequencing platforms.There are two main steps involved in 16S V3 – V4 amplicon processing: an initial PCR amplification using customized PCR primers that target the V3 and V4 domains, and a subsequent PCR amplification that integrates relevant flow cell binding domains and unique 12 base pair sample indices. The limited number of cleanup steps ensures maximum recovery of amplicons for downstream sequencing.
The NEXTFLEX® Poly(A) Beads 2.0 kit offers a convenient method for the purification of pure, intact mRNA upstream of RNA-Seq library prep with improved mRNA yields, low rRNA contamination, and a simple protocol.
The NEXTFLEX® 16S V4 Amplicon-Seq Library Prep Kit 2.0 is designed for the preparation of multiplexed amplicon libraries spanning the V4 hypervariable domain of microbial 16S ribosomal RNA (rRNA) genes. These libraries are compatible with paired-end sequencing on the Illumina® and Element Biosciences® sequencing platforms.