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CRISPRclean Stranded Total RNA Prep with rRNA Depletion (Human, Mouse, Rat)
The CRISPRclean® Stranded Total RNA Prep with rRNA Depletion (Human, Mouse, Rat) is a total RNA library prep leveraging a CRISPR-powered depletion workflow facilitating the detection of lower expressing and biologically relevant transcripts from complex samples.
For research use only. Not for use in diagnostic procedures.
Product information
Overview
Specific, unbiased rRNA depletion and library prep for RNA-Seq
In RNA samples, up to 90% consists of ribosomal RNA. Failing to remove these abundant rRNA sequences during transcriptome sequencing reduces sequencing capacity and hinders the detection of biologically relevant, low-expressing transcripts. Discover how CRISPRclean® enhances your research by efficiently removing rRNA.
Key highlights:
- Efficient multi-species depletion using CRISPR-Cas9 technology in a single tube.
- Enhances detection of biologically relevant, low-expressing transcripts.
- Our optimized library prep and depletion workflow ensures high-quality transcript representation.
- Achieve consistent full-length, uniform transcript coverage with high library complexity.
- Complete your workflow in just one day: 7.5 hours assay with 3 hours of hands-on time.
Additional product information
Figure 1: >90% rRNA depletion across mammalian species. Universal reference RNAs (Human, mouse, rat, chicken, cow, and dog) were used to evaluate the performance of ribodepletion across multiple species.
Multiplexing options
The NEXTFLEX™ RNA-seq 2.0 Unique Dual Index Barcodes (1-384) are provided at a concentration optimized for use with Jumpcode Genomics’ CRISPRclean ® Stranded Total RNA Prep with rRNA Depletion (Human, Mouse, Rat).
Specifications
| Shipping Conditions |
Dual Temperature
|
|---|---|
| Unit Size |
24 rxns
|
Resources
Datasheet
CRISPRclean™ Stranded Total RNA Prep with rRNA Depletion (Human, Mouse, Rat)
CRISPR-based ribodepletion strategy improves sensitivity for the detection of lower expressing transcripts from mammalian tissues
SDS, COAs, Manuals and more
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