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DepleteX High Expressing RNA Depletion Kit
Now you can enhance detection of low-expressing transcripts using the Jumpcode Genomics comprehensive RNA depletion panel. The DepleteX® High Expressing RNA Depletion kit selectively removes the most highly expressed transcripts in vitro before sequencing. This process reallocates approximately 70% of sequencing reads to unique, biologically relevant transcripts, enabling enhanced detection of low-expressing transcripts and rare isoforms.
For research use only. Not for use in diagnostic procedures.
Product information
Overview
Improve detection sensitivity in fibroblasts and whole blood.
While resequencing highly expressed transcripts contributes minimally to biologically relevant information, these transcripts dominate sequencing coverage, hindering detection of low-expressing genes, rare variants, and unique fusion events associated with diseases.
DepleteX® utilizes Cas9 and a guide RNA set to selectively remove 70% of the most highly expressed transcripts from poly-A selected RNA-Seq libraries obtained from fibroblasts and whole blood. This design optimizes the reallocation of sequencing reads, enhancing coverage of rare, low-expressing transcripts and unique fusion events.
Key highlights of the DepleteX® High Expressing RNA Depletion kit:
- Efficiently remove >4,000 highly expressed transcripts from whole blood and fibroblasts
- Allocate approximately 70% more reads to biologically relevant low-expressing transcripts.
- Enhance variant and isoform detection while minimizing sequencing requirements.
- Improved detection for low-expressing transcripts.
- Seamlessly integrate into your workflow using a straightforward and adaptable protocol
The DepleteX® High Expressing RNA Depletion kit is used during the RNA-seq library prep workflow starting with fibroblasts and whole blood samples after the ligation step and before the PCR step. This kit is compatible with most RNA-seq workflows; however, we recommend using it with the NEXTFLEX® Rapid Directional RNA-seq kit 2.0.
Specifications
| Shipping Conditions |
Shipped in Dry Ice
|
|---|---|
| Unit Size |
24 rxns
|
Resources
Enhanced RNA-Seq delivers double the genes detected with confidence
Please fill out this form below to access our list of highly expressed genes depleted using the DepleteX™ High Expressing RNA...
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