Improve detection sensitivity in fibroblasts and whole blood.

While resequencing highly expressed transcripts contributes minimally to biologically relevant information, these transcripts dominate sequencing coverage, hindering detection of low-expressing genes, rare variants, and unique fusion events associated with diseases.
DepleteX® utilizes Cas9 and a guide RNA set to selectively remove 70% of the most highly expressed transcripts from poly-A selected RNA-Seq libraries obtained from fibroblasts and whole blood. This design optimizes the reallocation of sequencing reads, enhancing coverage of rare, low-expressing transcripts and unique fusion events.

Key highlights of the DepleteX® High Expressing RNA Depletion kit:

• Efficiently remove >4,000 highly expressed transcripts from whole blood and fibroblasts
• Allocate approximately 70% more reads to biologically relevant low-expressing transcripts.
• Enhance variant and isoform detection while minimizing sequencing requirements.
• Improved detection for low-expressing transcripts.
• Seamlessly integrate into your workflow using a straightforward and adaptable protocol

The DepleteX® High Expressing RNA Depletion kit is used during the RNA-seq library prep workflow starting with fibroblasts and whole blood samples after the ligation step and before the PCR step. This kit is compatible with most RNA-seq workflows; however, we recommend using it with the NEXTFLEX^® Rapid Directional RNA-seq kit 2.0.