The DepleteX® Globin Depletion Kit selectively removes highly expressed globin transcripts (HBA1, HBA2, HBB, & HBD) in vitro before sequencing. This process redirects sequencing resources toward unique biologically relevant transcripts, maximizing UMI and gene sensitivity.
For research use only. Not for use in diagnostic procedures.
Enhance RNA-Seq quality by eliminating globin fragments.
Apart from ribodepletion, RNA samples often contain a limited number of well-characterized, highly expressed transcripts. However, resequencing these transcripts contributes minimally to biologically relevant information. The dominance of highly expressed transcripts hinders coverage for low-expressing counterparts, resulting in reduced sensitivity for detecting disease-related low-expressing genes, rare variants, and unique fusion events.
Globin mRNA dominates the mRNA content of red blood cells and whole blood. Three globlin genes (HBA1, HBA2 and HBB) constitute ≥30% of total mRNA from blood.
Key highlights:
Shipping Conditions |
Shipped in Dry Ice
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Unit Size |
24 rxns
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Remove contaminating globin fragments for RNA-Seq
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