Application | Type of sample | What do you want to remove? | Kit |
---|---|---|---|
Single cell RNA-seq | Any single cell sample. Both 5’ and 3’ gene expression libraries. | CRISPR-based removal of human rRNA, mtRNA, non-transcriptomics reads and (optional) non-variable genes to reallocate up to 50% of sequencing reads to informative clusters | DepleteX® Single Cell RNA Boost Kit |
Gene expression (Transcriptomics) | Human whole blood | Selective CRISPR-based removal of highly expressed globin transcripts (HBA1, HBA2, HBB, & HBD), maximizing UMI and gene sensitivity | DepleteX® RNA Depletion (Globin) Kit |
Human fibroblast or whole blood | CRISPR-based removal of common transcripts. Reallocates 70% of sequencing reads to biologically relevant transcripts, enhancing detection of low-expressing transcripts and rare isoforms | DepleteX® High Expressing RNA Depletion Kit | |
Human sample (any type) | CRISPR-based removal of human rRNA sequences, increasing representation of relevant transcripts | CRISPRclean® Stranded Total RNA | |
Human sample (any type) | Hybridization-based removal of human rRNA sequences, increasing representation of relevant transcripts | NEXTFLEX™ Ribonaut rRNA Depletion Kit | |
Human sample (any type) | CRISPR-based removal of human mitochondrial DNA. Capture more information from samples with high mitochondrial content | DepleteX® Mitochondrial DNA Kit | |
Metagenomics | Human origin (stool, saliva, etc) | CRISPR-based removal of human host rRNA sequences, increasing representation of relevant transcripts | CRISPRclean® Stranded Total RNA Kit |
Human origin (stool, saliva, etc) | CRISPR-based removal of human and bacterial rRNA sequences, increasing representation of relevant transcripts. | CRISPRclean® Plus Stranded Total RNA Kit | |
Human origin (nasopharyngeal swab) | CRISPR-based removal of human rRNA sequences, highly expressed human transcripts, and bacterial rRNA increasing representation of relevant transcripts. | DepleteX® Nasopharyngeal Microbial RNA Boost Kit |
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