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NEXTFLEX RiboNaut rRNA Depletion Kit (Human / Mouse / Rat)
The NEXTFLEX® RiboNaut™ rRNA depletion kit (human / mouse / rat) is an effective method to remove rRNA contamination from mammalian total RNA while enabling labs to interrogate additional RNA species in a sample, not only limited to intact mRNAs.
For research use only. Not for use in diagnostic procedures.
Product information
Overview
- Hybridization and bead-based protocol for rRNA depletion
- Optimized for use with 5 ng – 1 µg of total RNA as starting material
- Tested using the NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0 and automated on the Sciclone® G3 NGSx and Zephyr® G3 NGS workstations
- Versions are available for automated and manual rRNA depletion
The NEXTFLEX RiboNaut™ Kit Ensures Effective rRNA Depletion using Hybridization Technology
The NEXTFLEX RiboNaut rRNA depletion kit (human / mouse / rat) utilizes subtractive hybridization technology consisting of a mixture of biotinylated oligos complementary to rRNAs to pull them out of the sample using streptavidin-coated magnetic beads. The kit has been optimized for use with 5 ng – 1 µg of total RNA as starting material to deplete cytoplasmic and mitochondrial rRNAs. It has been verified to be compatible with human, mouse, and rat RNA and it may be compatible with other mammalian species.
Specifications
| Format |
Automated
|
|---|---|
| Unit Size |
48 rxns
|
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