Library Prep Kits

The NEXTFLEX® NGS Cleanup Beads are Solid-phase reversible immobilization (SPRI) beads that efficiently purify nucleic acids using reversible magnetic bead binding. They are ideal for PCR and NGS cleanup, removing unwanted components like soluble contaminants, nucleotides, primers, salts, and adapters. They selectively bind DNA by size with high recovery and consistent results. These SPRI beads can be used manually or in any automated liquid handler, depending on sample throughput.

The NEXTFLEX™ HT Agrigenomics Low-pass WGS kit is designed for the highly efficient conversion of DNA, from both high- and low-quality samples from breeders, into Illumina® and Element® compatible libraries and can be paired with analysis solutions from CURIO. Curio Genomics is a bioinformatics software company focused on accelerating agriculture genomics research.

The NEXTFLEX® Rapid XP V2 DNA-seq kit for Metagenomics offers a simplified DNA-seq library preparation workflow for whole genome sequencing (WGS) of microbial communities on Illumina® and Element Biosciences® platforms. Libraries produced using this kit are normalized during library construction using proprietary NEXTFLEX normalization beads, delivering consistent mass and sequencing cluster density for all samples in a library pool. The NEXTFLEX Rapid XP V2 DNA-seq kit for Metagenomics is now bundled with the CosmosID-HUB analysis solution, delivering user-friendly access to version-controlled pipelines for whole genome sequencing.

The NEXTFLEX™ UDI-UMI Barcodes consist of 96 unique dual-index (UDI) barcodes, each with a 10-base pair UDI and a 9-base pair unique molecular identifier (UMI). These UMI adapters are designed for multiplexing both DNA and RNA NGS libraries on all Illumina® and Element® sequencers and are compatible with any TruSeq® style library prep kit.

The DepleteX® Mitochondrial DNA Depletion kit employs a straightforward CRISPR-based method to selectively remove unwanted human molecules derived from mitochondrial nucleic acid before sequencing. Researchers can achieve more informative results while sequencing less in samples with abundant mitochondrial content such as ATAC-seq NGS libraries.

Revolutionize your RNA-seq analysis by purging excessive rRNA from human, mouse, rat, and bacterial samples. Our cutting-edge protocol is crafted for high-throughput applications by enabling depletion on multiplexed pools of samples.

Elevate your RNA-seq library quality by targeting and removing excessive rRNA from human, mouse, or rat samples. This advanced protocol is engineered for high-throughput screening, shifting the ribodepletion process to post-library preparation and facilitating efficient processing of up to 96 multiplexed libraries simultaneously.

The CRISPRclean® Stranded Total RNA Prep with rRNA Depletion (Human, Mouse, Rat) is a total RNA library prep leveraging a CRISPR-powered depletion workflow facilitating the detection of lower expressing and biologically relevant transcripts from complex samples.

Now you can enhance detection of low-expressing transcripts using the Jumpcode Genomics comprehensive RNA depletion panel. The DepleteX® High Expressing RNA Depletion kit selectively removes the most highly expressed transcripts in vitro before sequencing. This process reallocates approximately 70% of sequencing reads to unique, biologically relevant transcripts, enabling enhanced detection of low-expressing transcripts and rare isoforms.

The DepleteX® Single Cell RNA Boost Kit employs a CRISPR-based method to selectively remove unwanted reads allowing you to cut through the noise with minimal impact on your workflow and maximum confidence in your results.

The DepleteX® Nasopharyngeal Microbial RNA Boost Kit selectively removes highly expressed transcripts in vivo before sequencing. This process redistributes sequencing clusters toward microbial content and pathogens, enabling maximum discovery.

The DepleteX® Globin Depletion Kit selectively removes highly expressed globin transcripts (HBA1, HBA2, HBB, & HBD) in vitro before sequencing. This process redirects sequencing resources toward unique biologically relevant transcripts, maximizing UMI and gene sensitivity.

Jumpcode Genomics’ CRISPRclean™ Plus Stranded Total RNA Prep with rRNA Depletion (Human, Mouse, Rat, Pan Bacteria) kit is a directional RNA library prep leveraging a CRISPR-powered depletion workflow facilitating the detection of lower expressing and biologically relevant transcripts from complex samples. An optimized workflow with 9-hour total assay time and 3.5 hours of hands-on time (HOT) is made up of 7 simple steps with multiple safe stopping points.

NEXTFLEX Neo NGS RUO kits offer robust genome coverage to identify variants in over 390 genes.

The NEXTFLEX® blood miRNA blockers are an oligonucleotide mix which reduce the abundance of specific microRNA abundant in total RNA isolated from human whole blood and plasma in small RNA libraries. They are intended to be used with the NEXTFLEX® Small RNA-Seq Kit v4.

Library preparation kits for 16S V1-V3 rRNA bacterial metagenomics analysis for Illumina® and Element Biosciences® NGS platforms.

The NEXTFLEX® Rapid XP V2 DNA kit offers a simplified DNA library preparation workflow for sequencing on Illumina® and Element Biosciences® platforms. Libraries produced using this kit are normalized during library construction using proprietary NEXTFLEX® normalization beads, delivering consistent mass and sequencing cluster density for all samples in a library pool.

The DOPlify® WGA V2 kit performs fast whole genome amplification on single cells or limited template DNA samples, allowing for the cell chromosome copy number status to be determined.

The NEXTFLEX®  Cell Free DNA-Seq Kit 2.0 can produce libraries from 10 ng of cell free DNA for circulating tumor DNA (ctDNA) or cell free fetal DNA (cffDNA) analysis, for sequencing on Illumina® or Element Biosciences® NGS platforms in two hours.

The NEXTFLEX® RiboNaut™ rRNA depletion kit (human / mouse / rat) is an effective method to remove rRNA contamination from mammalian total RNA while enabling labs to interrogate additional RNA species in a sample, not only limited to intact mRNAs.

The NEXTFLEX® Unique Dual Index Barcodes are barcoded adapters that can be used with single read or paired-end read sequencing, and are compatible with NEXTFLEX library prep kits designed for sequencing on Illumina® and Element® platforms. They are compatible with any library prep workflows that involve the ligation of adapters to adenylated fragments (TruSeq® style).

The NEXTFLEX 16S V3 – V4 Amplicon-Seq Library Prep Kit is designed for the preparation of multiplexed amplicon libraries that span the hypervariable domains three through four (V3 and V4) of microbial 16S ribosomal RNA (rRNA) genes. These libraries are compatible with paired-end sequencing on the Illumina® and Element Biosciences® sequencing platforms.There are two main steps involved in 16S V3 – V4 amplicon processing: an initial PCR amplification using customized PCR primers that target the V3 and V4 domains, and a subsequent PCR amplification that integrates relevant flow cell binding domains and unique 12 base pair sample indices. The limited number of cleanup steps ensures maximum recovery of amplicons for downstream sequencing.

The NEXTFLEX® Universal Blockers are short DNA oligos block non-specific hybridization between adapter sequences, enhancing specificity and reducing sequencing costs. They are compatible with all NEXTFLEX barcodes and all other ligation and tagmentation based workflows for Illumina® and Element® sequencing platforms.

The NEXTFLEX® Poly(A) Beads 2.0 kit offers a convenient method for the purification of pure, intact mRNA upstream of RNA-Seq library prep with improved mRNA yields, low rRNA contamination, and a simple protocol.

The NEXTFLEX™ Rapid Directional RNA-seq library prep kit 2.0 produces libraries for Illumina® and Element Biosciences® sequencing instruments with high coverage uniformity, low duplication rates, strand specificity and minimal rRNA contamination when used with the NEXTFLEX Poly(A) Beads 2.0 (10 ng – 5 μg) or NEXTFLEX RiboNaut™ rRNA depletion kit (human, mouse, rat) (5 ng – 1 μg).