DOPlify Whole Genome Amplification V2 Kit

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DOPlify Whole Genome Amplification V2 Kit

The DOPlify^® WGA V2 kit performs fast whole genome amplification on single cells or limited template DNA samples, allowing for the cell chromosome copy number status to be determined.

For research use only. Not for use in diagnostic procedures.

Product information

SDS, COAs, Manuals and more

Product information

Overview

Single cell whole genome amplification

The DOPlify^® WGA V2 kit offers the advantage of full mitochondrial DNA coverage and the ability to add custom primers to specially amplify regions of interest. Products of the DOPlify^® WGA V2 kit can be used for a wide range of downstream processes, workflows, and NGS applications. The technology captures exclusive know-how for the lysis and whole genome amplification, with reagents provided in an easy-to-use kit format and less than 3 hour protocol time.

NGS compatible advanced DOP-PCR
Fast whole genome amplification with protocol time < 3 hours
Reduced contamination risk with only 2 tube openings required and 3 reagents to add
Can be fully automated with the JANUS® G3 liquid handler
No amplification of No Template Control
- 2.5 μg of DNA from one cell

Additional product information

Why Use DOPlify Technology?

The DOPlify WGA V2 kit is a whole genome amplification kit designed to PCR amplify the genome from single cells and limited DNA templates.

The DOPlify kit is unique in that it uses advanced DOP-PCR, which;

uses the high processivity and fidelity of new generation polymerases that are NGS application grade
uses ready-mixes to minimize the pipetting steps and requiring only two sample tube openings to reduce the risk of contamination
provides an amplification system that is flexible enough to concurrently whole genome amplify a sample while specifically amplifying genes of interest, reducing the risk of allele drop-out.

dopify whole genome amplification v2 kit

DOPlify workflow

Protocol Step	Explanation	Time
Cell Lysis	A gentle but effective enzyme-based lysis procedure ensures robust cell lysis and a readily accessible DNA template for whole genome amplification	15 mins
Whole Genome Amplification	Whole genome amplification is performed using advanced DOP-PCR, which has been optimized for limited DNA input. DOP-PCR uses degenerate primers to initiate DNA amplification, binding across a broad range of different sequences scattered genome wide	2.5 hrs
Quality Control	Following amplification, the analysis of products by agarose gel electrophoresis or microfluidic electrophoresis is recommended to ensure that cell amplification has been successful	30 mins

Can DOPlify WGA V2 be automated?

The whole genome amplification of single or small numbers of cells and downstream processes such as library preparation for NGS can be fully automated with the JANUS G3 liquid handler.

Whole genome amplification (WGA) of single cells in tubes has traditionally been performed by hand, which can lead to multiple potential issues including:

Errant volume pipetting
Technologist fatigue
Wasted time
Sample contamination

Using the JANUS G3 liquid handler with preprogrammed, easy-to-use automated WGA protocols your lab can:

Reduce human errors and contamination risk
Gain time for your staff to perform more important tasks
Increase your lab’s throughput with walkaway automation
Ensure sample process traceability with barcode scanning and sample tracking

Revvity’s JANUS G3 liquid handler can be used to automated whole genome amplification using the DOPlify kit or other WGA technologies with a throughput of 48 - 96* samples per run.

* Higher throughput options are available

Specifications

Other Specifications

Brand	DOPlify
Unit Size	50 rxn

References

Deleye, L., Tilleman, L., Plaetsen, A. V., Cornelis, S., Deforce, D., & Nieuwerburgh, F. V. (2017). Performance of four modern whole genome amplification methods for copy number variant detection in single cells. Scientific Reports, 7(1). doi:10.1038/s41598-017-03711-y.
Deleye, L., Plaetsen, A. V., Weymaere, J., Deforce, D., & Nieuwerburgh, F. V. (2018). Short Tandem Repeat analysis after Whole Genome Amplification of single B-lymphoblastoid cells. Scientific Reports,8(1). doi:10.1038/s41598-018-19509-5.
Mai, A. D., Harton, G. L., Quang, V. N., Van, H. N., Thi, N. H., Thuy, N. P., . . . Quoc, Q. T. (2020). Development and clinical application of a preimplantation genetic testing for monogenic disease (PGT-M) for beta thalassemia in Vietnam. Journal of Assisted Reproduction and Genetics. doi:10.1007/s10815-020-02006-y.
Plaetsen, A. V., Deleye, L., Cornelis, S., Tilleman, L., Nieuwerburgh, F. V., & Deforce, D. (2017). STR profiling and Copy Number Variation analysis on single, preserved cells using current Whole Genome Amplification methods. Scientific Reports, 7(1). doi:10.1038/s41598-017-17525-5.