NEXTFLEX Rapid XP V2 DNA-Seq Kit for Metagenomics

Analysis Powered by CosmosID-HUB®

The NEXTFLEX Rapid XP v2 Kits are now available bundled with the CosmosID-HUB® analysis platform; an online software solution that enables fast and easy analysis of complex microbiome data. CosmosID-HUB gives scientists user-friendly access to two cutting-edge version-controlled and validated metagenomic pipelines such as KEPLER™ and CHAMP™. The software also enables rapid data interpretation through a comparative analysis software with features such as tables, heatmaps, bar charts, multiple Alpha & Beta Diversity indexes, abundance distribution plots, differential abundance testing, as well as comprehensive statistics between groups. Learn more about CosmosID-HUB.

CosmosID Benefits include:

• Save costs on computational infrastructure
• Save time and get profiling data within hours
• Centralized and integrated data storage for ease of access
• Create virtual cohorts with your data and compare microbiome data in minutes
• Scale your team with easy to interpret charts and visualization
• No coding experience required.
• 100+ citations utilizing these pipelines

KEPLER™ Host-Agnostic Microbiome Profiler:

Kepler employs a versatile, host-independent curated database to analyze a wide range of samples, including environmental, animal, soil, and food samples, among others. Kepler maximizes the value of metagenomic data by integrating the accuracy of K-mer exact-matching with the flexibility of probabilistic alignment. This approach enables Kepler to effectively identify and quantify bacteria, viruses, fungi, and protists, utilizing a carefully curated biomarker database that organizes over 30,000 species in a phylogenetic tree-like structure

Benefits of KEPLER:

• Kepler can analyze microbiome data without being limited to specific host organisms, making it versatile for various research applications.
• The pipeline is designed to detect low-abundance and rare species accurately, ensuring precise identification of microorganisms present in the samples.
• Kepler provides reliable measurements of species abundance, which is crucial for understanding the microbial composition of samples.
• It offers detailed taxonomic annotation at the species level, enhancing the accuracy of microbiome profiling.

CHAMP™ Human Microbiome Profiler

By integrating the most extensive reference catalogue for human-associated microbes with a unique algorithm for species detection and abundance estimation, CHAMP™ delivers superior microbiome data for further analysis.

Whether you’re analyzing low biomass samples, searching for rare species, profiling the microbiome in large-scale population health studies, or designing next-generation probiotics or live biotherapeutics, CHAMP™ sets new benchmarks in sensitivity and accuracy for microbiome research.

CHAMP benefits:

• Identifies low-abundance and rare species within samples.
• Detects only the species that are truly present in the samples.
• Delivers accurate measurements of species abundance.
• Accurately annotates microorganisms at the species level

Additionally, CHAMP™ offers phage and virome profiling along with advanced functional annotations. With the inclusion of our clonal-level analysis tool, it can distinguish very closely related strains, aiding researchers in understanding the interactions between probiotics/therapeutic microbes and native populations of the same species

Highly Efficient Ligation with Low Adapter Formation

The NEXTFLEX Rapid XP v2 DNA-Seq Kit is designed to provide exceptional ligation efficiency, which is crucial for high-quality sequencing results. The kit includes optimized reagents and protocols that ensure the efficient and accurate ligation of DNA fragments. This high ligation efficiency reduces the likelihood of adapter dimers or erroneous sequences even at low input concentrations, leading to more reliable and reproducible data. By maximizing the ligation step, the kit enhances overall workflow efficiency and contributes to the generation of high-quality sequencing libraries.

Included Normalization Beads provide highly consistent results while saving you several hours of time while preparing for sequencing

Normalization Beads offer significant benefits in DNA sequencing workflows by ensuring consistent and accurate sample preparation. They streamline the process by equalizing the concentration of DNA across different samples, which reduces variability and enhances the reliability of sequencing results. This leads to more efficient and cost-effective sequencing, as it minimizes the need for additional normalization steps and reduces the risk of errors. Overall, Normalization Beads contribute to higher quality data and more reproducible outcomes.

Flexible multiplexing options: Up to 1,536 UDI or 96 UDI-UMI adapters

Barcodes are not supplied with the NEXTFLEX Rapid XP DNA-seq kit v2 and need to be purchased separately. We offer 384 color-balanced NEXTFLEX UDI adapters that improve multiplexing capabilities for both low-level and high-level multiplexing needs and a set of 1,536 NEXTFLEX UDI adapters for ultra high-throughput applications.

Automation Compatibility

The NEXTFLEX Rapid XP V2 DNA-Seq kit is compatible with multiple liquid handlers, including the Sciclone G3 NGSx workstation, Sciclone G3 NGSx iQ™ workstation, and Zephyr NGS workstation

Workflow

Listen to the experts.

Watch Britton Strickland, Ph.D., and Gustav Lindved in the webinar, Advanced, End-To-End Shotgun Metagenomics Workflows, as they introduce the CosmosID-HUB and how it enables the understanding of the functional roles of microbes in various environments.

Watch our on-demand webinar now.

Revvity Shotgun Analysis - Online Demonstration

Now you can schedule a one-on-one 30-minute online demonstration that will cover everything you need to know about the CosmosID-Hub® which is now available with Revvity’s NEXTFLEX™ Rapid XP V2 DNA-Seq kit for Metagenomics. You will be able to see the analysis demonstrated and our experts will be able to answer any questions that you may have. Please choose a timeframe that suits you best and we will provide you a Microsoft Teams personal link to join when the time comes. We look forward to speaking with you.