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NEXTFLEX NGS Cleanup Beads

The NEXTFLEX® NGS Cleanup Beads are Solid-phase reversible immobilization (SPRI) beads that efficiently purify nucleic acids using reversible magnetic bead binding. They are ideal for PCR and NGS cleanup, removing unwanted components like soluble contaminants, nucleotides, primers, salts, and adapters. They selectively bind DNA by size with high recovery and consistent results. These SPRI beads can be used manually or in any automated liquid handler, depending on sample throughput.

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Feature Specification
Automation Compatible Yes
Product Group Accessories

The NEXTFLEX® NGS Cleanup Beads are Solid-phase reversible immobilization (SPRI) beads that efficiently purify nucleic acids using reversible magnetic bead binding. They are ideal for PCR and NGS cleanup, removing unwanted components like soluble contaminants, nucleotides, primers, salts, and adapters. They selectively bind DNA by size with high recovery and consistent results. These SPRI beads can be used manually or in any automated liquid handler, depending on sample throughput.

Click to copy promo code to clipboard.
img-icon-10-off-white-yellow.svg

SAVE 10% on your first Revvity.com order. Use promo code below.

HELLO10

Terms and conditions apply.

Product Variants
Unit Size: 1 mL
Part #:
NOVA-BEADS-1ML
List Price
USD 8.00
Your online price:
Unit Size: 5 mL
Part #:
NOVA-BEADS-5ML
List Price
USD 40.00
Your online price:
Unit Size: 50 mL
Part #:
NOVA-BEADS-50ML
List Price
USD 400.00
Your online price:
Unit Size: 500 mL
Part #:
NOVA-BEADS-500ML
List Price
USD 4,000.00
Your online price:
For research use only. Not for use in diagnostic procedures.
Total list price:
USD 400.00
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Overview

The NEXTFLEX NGS Cleanup Beads are a cost-effective solution for these applications:

  • NGS Library Prep protocols
  • Any protocols requiring the removal of unwanted components such as primers, salts or size selection of DNA
  • Drop-in replacement for most SPRI beads


Important Attributes:

  • Input: Amplicons, fragmented gDNA
  • Applications: PCR Purification, DNA Cleanup, NGS Cleanup
  • Recovery: 70-90% for dsDNA >100 bp
  • Size Selection: Please check what Bead-to-sample Ratio is and why it matters
  • Storage: 4ºC

Additional product information

NEXFLEX-Cleanup-Beads-Figure


Performance

Figure 1 shows an example of size selection performed with NEXTFLEX® NGS Cleanup and a popular competitor using different bead-to-sample ratios. Results show a predictable and consistent size selection, comparable to competition.

Bead-Comparison


Figure 1. 625 ng of 50 bp DNA ladder (Promega) were purified with equal amount of AMPure® XP (Beckman) and NEXTFLEX® NGS Cleanup and run on 1% agarose gel for comparison.

Bead-Chart


Figure 2. shows final yields obtained when using same bead-to-sample ratio of either the NEXTFLEX® NGS Cleanup or a popular competitor to cleanup the libraries generated with our NEXTFLEX 16S V4 2.0 kit. Yields of both solutions are not significantly different

Specifications

Automation Compatible
Yes
Product Group
Accessories
Shipping Conditions
Shipped Ambient
Unit Size
50 mL

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Efficient DNA purification using SPRI technology

This flyer describes showcases the NEXTFLEX NGS Cleanup Bead specifications and uses cases.

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