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NEXTFLEX Cell Free DNA-Seq Library Prep Kit 2.0
The NEXTFLEX® Cell Free DNA-Seq Kit 2.0 can produce libraries from 10 ng of cell free DNA for circulating tumor DNA (ctDNA) or cell free fetal DNA (cffDNA) analysis, for sequencing on Illumina® or Element Biosciences® NGS platforms in two hours.
For research use only. Not for use in diagnostic procedures.
Product information
Overview
- Designed for low sample input – 10 ng of input cfDNA required
- Accelerated workflow with minimized hands-on time
- Flexible adapter barcode options – up to 1,536 unique dual index barcodes are available
- Compatible with Illumina® sequencing platforms
- Protocols available for automation on the Sciclone® G3 NGSx, NGSx iQ™ and Zephyr® G3 NGS workstations
The NEXTFLEX® Cell Free DNA-Seq Kit 2.0 delivers high coverage and reduced bias for Illumina® sequencing applications. The NEXTFLEX® Cell Free DNA-Seq Kit 2.0 can be used to prepare single, paired-end and multiplexed DNA libraries for sequencing using Illumina® platforms. The availability of up to 1,536 unique dual index barcodes facilitates high-throughput applications without compromising data integrity.
Reduced Bias and Coverage Gaps
NEXTFLEX® Cell Free DNA-Seq Kit 2.0 exhibits minimal GC bias and produces uniform coverage of difficult to sequence genome regions. This kit also offers robust ligation efficiency, resulting in greater library diversity and a larger number of unique sequencing reads. These optimizations enable investigators to obtain more information from each sequencing run.
Flexible Multiplexing Options
The NEXTFLEX® Cell Free DNA-Seq Library Prep Kit 2.0 is designed to be used with the NEXTFLEX® Unique Dual Index barcodes (384 barcodes) or the NEXTFLEX® Unique Dual Index barcodes (1,536 barcodes).
Specifications
| Brand |
NEXTFLEX
|
|---|---|
| Shipping Conditions |
Dual Temperature
|
| Unit Size |
48 rxns
|
Resources
Benefit of an automated cfDNA analysis workflow
The presence of double stranded, circulating cell free DNA (cfDNA) in blood plasma...
SDS, COAs, Manuals and more
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