HTRF

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HTRF™ (Homogeneous Time Resolved Fluorescence) is a no-wash technology that combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence.

This technology can be utilized in cellular or biochemical assays, employing competitive and non-competitive formats. It may be used for drug discovery, biotherapy and basic research in therapeutic areas such as oncology, CNS and metabolic and immune disorders. Researchers can explore a wide range of applications, including GPCRs, kinases, epigenetics, biotherapy, and quantification of a range of biomarkers and cytokines.

Easy to perform: homogeneous assay with no wash requirement
High level of sensitivity, signal stable in time
Time and labor savings, rapid and accurate results
Amenable to miniaturization
Ability to detect and analyze small and large complexes with low to high affinity interactions
Field of applications: basic research, target discovery and validation, HTS and primary screening, secondary screening, lead optimization

For research use only. Not for use in diagnostic procedures.

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Target area

Explore cell-based and biochemical assays by target area of interest, including cell and gene therapy, GPCRs, targeted protein degradation, and more.

Biologics screening and characterization: Explore assays for biologics screening, mechanism-of-action studies, and biomanufacturing.
Biomarker detection: Accurate detection and quantitation of molecular targets across serum, plasma, culture supernatants, cellular lysates, and other sample types.
Cell and gene therapy: Discover and advance cell and gene therapy research with bioanalytical immunoassays for CGT development.
Cell signaling: Focus on cell signaling and intracellular phosphorylated proteins, with streamlined approaches to pathway analysis.
Cytokines: Access a comprehensive range of cytokine assay kits, including interleukins, interferons, chemokines, tumor necrosis factors, and lymphokines.
Epigenetics: Leverage our epigenetics toolbox, designed to detect specific methylation and acetylation marks on histone peptide substrates.
GPCRs: Measure cAMP levels and track downstream protein phosphorylation with functional GPCR assays.
Protein kinases: Explore an extensive portfolio of protein phosphorylation assays, available in cell-based or biochemical no-wash formats.
Protein-protein interactions: Utilize highly sensitive assays to study protein interactions involved in a range of biological processes.
Receptor binding: Access cell-based solutions with no separation steps and eliminate radioactive waste, an improvement over traditional SPA and radioligand binding assays.
Targeted protein degradation: Monitor protein expression levels and characterize the mechanism of action using our total and phospho kits.

Therapeutic area

Explore cell-based and biochemical assays by your therapeutic area of research, including oncology, neuroscience, metabolic diseases, and more.

Autoimmune diseases: Comprehensive reagents for autoimmune disease research, including rheumatoid arthritis, lupus, inflammatory bowel disease, multiple sclerosis, and more.
Obesity, diabetes, and metabolic diseases: Solutions for drug discovery in obesity, diabetes, and metabolic diseases, featuring assays for biomarker detection, protein analysis, and GPCR research.
Fibrosis: Ready-to-use assays for monitoring biomarkers, phosphoproteins, and transcription factors relevant to fibrotic disease research.
Immuno-oncology: Assays for studying immunity and immune cells, covering protein, biomarker, and cytokine detection, as well as cell toxicity and proliferation.
Oncology: A comprehensive oncology portfolio, including assays for cytokines, biomarkers, phospho-proteins, cell proliferation, cytotoxicity, and more.
Neuroscience: A wide range of neuroscience assays to support research in neurodegenerative disorders such as Alzheimer’s, Parkinson’s, and Huntington's disease.
Virology: Assay technologies for viral studies, enabling assessment of viral life cycles and antiviral immune responses with streamlined workflows.