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HTRF™ (Homogeneous Time Resolved Fluorescence) is a no-wash technology that combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence.

This technology can be utilized in cellular or biochemical assays, employing competitive and non-competitive formats. It may be used  for drug discovery, biotherapy and basic research in therapeutic areas such as oncology, CNS and metabolic and immune disorders. Researchers can explore a wide range of applications, including GPCRs, kinases, epigenetics, biotherapy, and quantification of a range of biomarkers and cytokines.

  • Easy to perform: homogeneous assay with no wash requirement
  • High level of sensitivity, signal stable in time
  • Time and labor savings, rapid and accurate results
  • Amenable to miniaturization
  • Ability to detect and analyze small and large complexes with low to high affinity interactions
  • Field of applications: basic research, target discovery and validation, HTS and primary screening, secondary screening, lead optimization 

For research use only. Not for use in diagnostic procedures.
 

HTRF

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Target Area

Target area

Explore cell-based and biochemical assays by target area of interest, including cell and gene therapy, GPCRs, targeted protein degradation, and more.

Explore cell-based and biochemical assays by target area of interest, including cell and gene therapy, GPCRs, targeted protein degradation, and more.

  • Biologics screening and characterization: Explore assays for biologics screening, mechanism-of-action studies, and biomanufacturing.
  • Biomarker detection: Accurate detection and quantitation of molecular targets across serum, plasma, culture supernatants, cellular lysates, and other sample types.
  • Cell and gene therapy: Discover and advance cell and gene therapy research with bioanalytical immunoassays for CGT development.
  • Cell signaling: Focus on cell signaling and intracellular phosphorylated proteins, with streamlined approaches to pathway analysis.
  • Cytokines: Access a comprehensive range of cytokine assay kits, including interleukins, interferons, chemokines, tumor necrosis factors, and lymphokines.
  • Epigenetics: Leverage our epigenetics toolbox, designed to detect specific methylation and acetylation marks on histone peptide substrates.
  • GPCRs: Measure cAMP levels and track downstream protein phosphorylation with functional GPCR assays.
  • Protein kinases: Explore an extensive portfolio of protein phosphorylation assays, available in cell-based or biochemical no-wash formats.
  • Protein-protein interactions: Utilize highly sensitive assays to study protein interactions involved in a range of biological processes.
  • Receptor binding: Access cell-based solutions with no separation steps and eliminate radioactive waste, an improvement over traditional SPA and radioligand binding assays.
  • Targeted protein degradation: Monitor protein expression levels and characterize the mechanism of action using our total and phospho kits.
Therapeutic Area

Therapeutic area

Explore cell-based and biochemical assays by your therapeutic area of research, including oncology, neuroscience, metabolic diseases, and more.

Explore cell-based and biochemical assays by your therapeutic area of research, including oncology, neuroscience, metabolic diseases, and more.

  • Autoimmune diseases: Comprehensive reagents for autoimmune disease research, including rheumatoid arthritis, lupus, inflammatory bowel disease, multiple sclerosis, and more.
  • Obesity, diabetes, and metabolic diseases: Solutions for drug discovery in obesity, diabetes, and metabolic diseases, featuring assays for biomarker detection, protein analysis, and GPCR research.
  • Fibrosis: Ready-to-use assays for monitoring biomarkers, phosphoproteins, and transcription factors relevant to fibrotic disease research.
  • Immuno-oncology: Assays for studying immunity and immune cells, covering protein, biomarker, and cytokine detection, as well as cell toxicity and proliferation.
  • Oncology: A comprehensive oncology portfolio, including assays for cytokines, biomarkers, phospho-proteins, cell proliferation, cytotoxicity, and more.
  • Neuroscience: A wide range of neuroscience assays to support research in neurodegenerative disorders such as Alzheimer’s, Parkinson’s, and Huntington's disease.
  • Virology: Assay technologies for viral studies, enabling assessment of viral life cycles and antiviral immune responses with streamlined workflows.

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1 - 25 of 1217 Products and Services
New
Nucleolin Phospho-Thr84 Product image

This HTRF kit allows for the cell-based quantitative detection of Nucleolin when phosphorylated at Thr84.

Part Number: 64NCLT84PEG, 64NCLT84PEH
List price:
USD 2,147.00 - 12,490.00
New
Total B-RAF Product image

This HTRF kit allows for the cell-based quantitative detection of total B-RAF.

Part Number: 64BRAFTPEG, 64BRAFTPEH
List price:
USD 2,147.00 - 12,490.00
New
Total YES Product image

This HTRF kit allows for the cell-based quantitative detection of Total YES.

Part Number: 64YESTPEG, 64YESTPEH
List price:
USD 2,147.00 - 12,490.00
New
Total Nucleolin Product image

This HTRF kit allows for the cell-based quantitative detection of total Nucleolin.

Part Number: 64NCLTPEG, 64NCLTPEH
List price:
USD 2,147.00 - 12,490.00
New
LRRK2 Phospho-Ser1292 Product-image

This HTRF kit allows for the cell-based quantitative detection of LRRK2 when phosphorylated at Ser1292.

Part Number: 64LRRKS1PEG, 64LRRKS1PEH
List price:
USD 2,147.00 - 12,490.00
New
Total LRRK2 Product image

This HTRF kit allows for the cell-based quantitative detection of Total LRRK2.

Part Number: 64LRRKTPEG, 64LRRKTPEH
List price:
USD 2,147.00 - 12,490.00
New
NPM1 Phospho-Thr199 Product image

This HTRF kit allows for the cell-based quantitative detection of NPM1 when phosphorylated at Thr199.

Part Number: 64NPMT1PEG, 64NPMT1PEH
List price:
USD 2,147.00 - 12,490.00
New
IRAK4 Phospho-Thr345 Product image

This HTRF kit allows for the cell-based quantitative detection of IRAK4 when phosphorylated at Thr345.

Part Number: 64IRK4T45PEG, 64IRK4T45PEH
List price:
USD 2,147.00 - 12,490.00
New
CXCL12 Product image

The HTRF CXCL12 kit allows for the simple and rapid quantification of soluble CXCL12 in cell supernatants, providing a fast and no-wash alternative to traditional wash-based ELISA assays. The kit is suitable for the measurement of CXCL12 from both human and mouse cell lines.

Part Number: 62CXCL12PEG, 62CXCL12PEH
List price:
USD 1,103.00 - 10,290.00
New
Total NPM1 Product image

This HTRF kit allows for the cell-based quantitative detection of Total NPM1.

Part Number: 64NPMTPEG, 64NPMTPEH
List price:
USD 2,147.00 - 12,490.00
New
YES Phospho-Tyr426 Product image

This HTRF kit allows for the cell-based quantitative detection of YES when phosphorylated at Tyr426.

Part Number: 64YESY4PEG, 64YESY4PEH
List price:
USD 2,147.00 - 12,490.00
New
WRN Product image

This HTRF kit allows for the cell-based quantitative detection of WRN.

Part Number: 64WRNTPEG, 64WRNTPEH
List price:
USD 1,372.00 - 14,590.00
New
TNF alpha Product image

The HTRF HP Human TNF-α kit is designed for the simple and rapid quantification of TNF-α proteins in cell supernatants, offering a no-wash alternative to ELISA. This kit is a new and improved version of the existing kit, 62HTNFAPEG.

List price:
USD 652.00 - 10,290.00
New
Total A-RAF Product image

This HTRF kit allows for the cell-based quantitative detection of total A-RAF.

Part Number: 64ARAFTPEG, 64ARAFTPEH
List price:
USD 2,147.00 - 12,490.00
New
CCL17 Product image

The HTRF human CCL17 kit is for the simple and rapid quantification of soluble human CCL17 proteins in cell culture supernatants, and can be used as a no-wash alternative to more traditional wash-based ELISA.

Part Number: 62HCCL17PEG, 62HCCL17PEH
List price:
USD 1,103.00 - 10,290.00
Picture anti-FLAG XL665

XL665-labeled anti-FLAG antibody for capturing FLAG-tagged proteins in protein/protein interaction assays.

Part Number: 61FGBXLF, 61FGBXLA, 61FGBXLB
List price:
USD 262.00 - 1,911.00
Picture CK1a total kit

This HTRF kit allows the cell-based quantitative detection of Total CK1a.

Part Number: 64CK1ATPEG, 64CK1ATPEH
List price:
USD 2,147.00 - 12,490.00
Picture SALL4 total kit

This HTRF kit allows for enable the cell-based quantitative detection of Total SALL4.

Part Number: 64SALL4TPEG, 64SALL4TPEH
List price:
USD 2,147.00 - 12,490.00
Picture RNA pol 2 phospho-Ser5 kit

This HTRF kit Allows for the cell-based quantitative detection of RNA Pol II when phosphorylated at Ser5.

Part Number: 64RNAP2S5PEG, 64RNAP2S5PEH
List price:
USD 2,147.00 - 12,490.00
Picture RNA pol 2 total kit

This HTRF kit enables the cell-based quantitative detection of Total RNA Pol II

Part Number: 64RNAP2TPEG, 64RNAP2TPEH
List price:
USD 2,147.00 - 12,490.00
Picture RNA pol 2 phospho Ser2 kit

This HTRF kit allows for the cell-based quantitative detection of RNA Pol II when phosphorylated at Ser2.

Part Number: 64RNAP2S2PEG, 64RNAP2S2PEH
List price:
USD 2,147.00 - 12,490.00
Picture of HTRF dsRNA IVT kit

The HTRF dsRNA (IVT) kit is intended for the simple and rapid quantification of double-stranded RNA (dsRNA) particles in samples resulting from In Vitro Transcription (IVT) of mRNA. It offers a fast and homogeneous alternative to ELISA or immunoblot.

Part Number: 64DSRNAPEG, 64DSRNAPEH
List price:
USD 2,524.08 - 29,681.60
Picture of HTRF MerTK phospho-Y749 kit

This HTRF kit allows for the cell-based quantitative detection of MerTK when phosphorylated at Tyr749.

Part Number: 64MERTK49PEG, 64MERTK49PEH
List price:
USD 2,271.53 - 13,214.42
Picture of HTRF MerTK total kit

This HTRF kit allows for the cell-based quantitative detection of Total MerTK.

Part Number: 64MERTKTPEG, 64MERTKTPEH
List price:
USD 2,271.53 - 13,214.42
Picture of HTRF ATF4 total kit

This HTRF kit enables the cell-based quantitative detection of Total ATF4.

Part Number: 64ATF4TPEG, 64ATF4TPEH
List price:
USD 2,271.53 - 13,214.42
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Infographic
Infographic
A brief history on protein-protein interactions

Proteins and their interactions have a history of keen research and technological innovations. All scientists deciphering the protein interactions today are the heirs to this history and are now contributing to it, how well do you know it? Learn more in this infographic! For research use only. Not for use in diagnostic procedures.

Whitepaper
Whitepaper
A guide to In Vitro Transcription (IVT) and the importance of dsRNA detection

Application Note
Application Note
A guideline for HTRF cell-based phospho-protein data normalization

Get the best out of your phosphorylation assays Combining phospho and total protein assays enables better analysis. This Application Note provides valuable guidelines for efficiently analyzing and interpreting results from such assay combinations. Check out all the tips and examples in features! Features Introduction to phospho and total protein assay relevance Tips for handling and interpreting data Examples from actual experiments

Technical Note
Technical Note
A simple method for preparing GPCR membrane model extracts from stable cell lines for use with the HTRF GTP Gi binding assay

G-protein coupled receptors (GPCRs) are crucial transmembrane proteins involved in cellular signal transduction. This technical note outlines a method for preparing GPCR membrane model extracts from stable cell lines, specifically for use with the HTRF GTP Gi binding assay. Get this technical note and discover: Key Highlights such as the Importance of GPCRs and the advantages of using HTRF GTP Gi Binding Assay Detailed Method with Cell Culture Preparation, Cell Lysis, Membrane Preparation and Assay Optimization For research use only. Not for use in diagnostic procedures.

Infographic
Infographic
Addressing the interactome with protein-protein interaction assays

Protein-protein interactions: cover them all with one approach This brochure illustrates the possibilities and versatility of protein-protein interaction studies. It features six relevant examples of various interaction types taken from literature to show you how studies can be handled with the time-resolved fret-based HTRF approach, including virus blockade, receptor/ligand binding, protease activity, and more. Features: Introduction to the stakes of protein-protein interaction research Illustration of 6 published interaction studies involving biologics or small molecules For research use only. Not for use in diagnostic procedures.

Whitepaper
Whitepaper
Adeno-Associated Virus (AAV) Vectors Manufacturing

What Are the Challenges and Solutions? All across the globe, AAVs are getting the attention of scientists and companies working in gene therapy, as they provide the right combination of characteristics that make them one of the most promising vector today. In 2021 only, the global gene therapy market was 4.1 billion USD, and AAV vector therapies made up more than 43% of that market value! Designing and manufacturing AAV vectors is complex, and to be successful, certain challenges must be addressed. This implies monitoring and optimtizing production for a thorough quality control process, including reliable ongoing characterization of process intermediates and the final product. Key features include: Overview and data on the gene therapy market Description of AAV vectors’ genomes and transfer Challenges associated with AAV vectors’ design and manufacturing Analytical methods for AAV vector quality control with a description of innovative no-wash AlphaLISA™ assays

Flyer
Flyer
Advance your biologics research with Revvity solutions

In the dynamic landscape of pharmaceuticals, biotherapeutics research stands out as the fastest-growing sector. Scientists worldwide are on a quest for ever more efficient tools to develop therapeutic proteins. From lead selection to bioprocessing qualification, rigorous analytical characterization is essential. That’s where Revvity steps in. Our comprehensive line of no-wash tools revolutionizes biologics screening, mechanism-of-action studies, and biomanufacturing. With robustness and convenience at the forefront, we empower researchers to accelerate discoveries and drive innovation. Welcome to a new era of precision and efficiency in biotherapeutics research. For research use only. Not for use in diagnostic procedures.

Application Note
Application Note
Advancing K-Ras Targeted Therapies with Innovative Detection Methods

The RAS family of genes, particularly K-Ras, plays a critical role in cancer biology. Despite its notorious difficulty as a therapeutic target, recent breakthroughs have brought new hope in treating cancers driven by K-Ras mutations. Our latest application note delves into innovative approaches to K-Ras inhibition, including small molecule inhibitors, synthetic lethality strategies, and PROTAC® molecules. We also showcase the high specificity and sensitivity of the no-wash HTRF™ K- Ras immunoassay, a cutting-edge tool that offers a reliable and precise method for evaluating K-Ras protein levels, outperforming traditional techniques. Discover how this assay can accelerate your research in targeting the elusive K-Ras.

Application Note
Application Note
Advantages of using our automatable and no-wash HTRF and AlphaLISA kits for CHO HCP detection

Detecting and quantifying HCPs with automatable homogeneous immunoassays During biotherapeutic manufacturing and production, the host cells - a great majority of them being Chinese Hamster Ovary (CHO) cells – produce protein impurities that are called Host Cell Proteins (HCPs). Even if more than 99% of them are removed from the final product, the residual CHO HCPs can induce immunogenicity in individuals or reduce the potency, stability, or effectiveness of a drug. Therefore, to meet regulatory organizations’ guidelines (such as FDA or EMA) on CHO HCP levels, biopharmaceutical companies spend significant amounts of money on tools and strategies for their detection. Illustrated with robust results, this Application Note explains the many ways in which HTRF™ and AlphaLISA™ kits can improve the workflow for CHO HCP detection: Wide antibody coverage Compatibility with most commonly used buffers No cross-reactivity between CHO HCP detection and drug substance Good dilutional linearity and antigen spike recovery

Whitepaper
Whitepaper
An overview of atherosclerosis

Atherosclerosis pathogenesis, cellular actors, and pathways Atherosclerosis is a common condition in which arteries harden and become narrow due to a build-up of fatty material, usually cholesterol, and other substances such as calcium. This can lead to a range of serious health complications, including heart attack or stroke, making the disease an important contributing factor in death and morbidity in developed countries. Recent developments in our understanding of atherosclerosis from a molecular perspective include the discovery of new players in disease pathogenesis. Included in this white paper Atherosclerosis: step-by-step pathogenesis, therapeutic strategies, and recent developments Detailed descriptions and explanations, including a focus on pathways

Whitepaper
Whitepaper
Antibody-based therapeutic modalities in oncology

With over 200 different types of cancer, management relies on a variety of techniques such as chemotherapy, radiotherapy, and surgery. These types of therapies can be associated with severe side effects, and finding safer and more effective treatments is a priority in cancer therapeutics research. One approach that has shown huge potential is monoclonal antibody-based cancer therapeutics. In this white paper we explore this exciting area of anti-cancer research, covering mechanism of action, development, and challenges in monoclonal antibody-based therapeutics, including antibody-drug conjugates and multispecific antibodies.

Application Note
Application Note
Assessment of drug efficacy and toxicity by combining innovative technologies

Compound MoA and potential cytotoxic effects deciphered thanks to immunoassays and cell viability assay The attrition of drug molecules occurs at various stages in the development process, and most early-phase failures are attributed to safety and toxicity issues. Considering the economic impact of early project termination, the biggest challenge comes from selecting the most potent and selective drug compounds while assessing their potential toxic side effects. In this application note, you’ll discover how to easily combine AlphaLISA™, HTRF™, or AlphaLISA™ SureFire® Ultra™ immunoassays with the ATPlite™ 1step cell viability assay to simultaneously, and in a single sample, study the efficacy of drug compounds on disease pathways while identifying possible cytotoxic effects. Recommendations Detailed workflows Case studies on various cell lines and different markers using HTRF, AlphaLISA, or AlphaLISA SureFire Ultra immunoassays together with the ATPlite 1step cell viability assay

eBook
eBook
Autophagy regulation eBook

Taking autophagy regulation research a step further Autophagy regulation is a key molecular process involved in recycling long-lived protein and organelles. Dysregulation of autophagy leads to different pathologies such as cancer, neurodegenerative and infectious diseases. This eBook features: Key facts about autophagy and mitophagy Infographics to apprehend the basics Cutting-edge knowledge

Guide
Guide
Benefit from a collection of important NAFLD pathways

Get a useful overview of today’s NAFLD knowledge with this booklet. NASH disease is complex and follows many development pathways. This booklet provides you with critical information regarding NAFLD and more specifically about NASH progression. Review the fundamentals of NAFLD and NASH learn from an important research report Benefit from additional content to help your NASH research

Guide
Guide
Benefit from an insight into the diversity of immune cells & signaling pathways

Get a useful overview of today’s immunity knowledge with this booklet Immunity is a collection of complex processes involving multiple strategies and specialized cell types. This booklet provides you with critical information regarding their roles, characteristic and signaling pathways as well as the collaborative behaviors that contribute to immunity. Featured in this guide: Review the fundamentals of immune cell types and mechanisms Learn from a cutting-edge research report Pathways and functional details on over 10 specialized immune cells

Application Note
Application Note
CDK signaling in oncology: assess the effectiveness of a CDK9-targeting PROTAC molecule

CDK9 is an attractive target for cancer therapeutics due to its crucial role in transcription regulation, particularly of short-lived anti-apoptotic proteins such as MCL-1 and XIAP which are critical to the survival of cancer cells. One approach for specifically degrading CDK9 is using a proteolysis targeting chimera (PROTAC). In this application note, you will discover: The rapid and strong effect of CDK9 PROTAC molecule Thal-SNS-032, demonstrating specificity in CDK9 degradation How HTRF ® and AlphaLISA ® SureFire ® Ultra ™ are used to measure protein levels, with each tool showcasing the ability of PROTACs to rapidly degrade a targeted protein making it a unique tool in the fight against cancer

Application Note
Application Note
CDK signaling: Investigate CDK4/6 inhibition in breast cancer cell lines with HTRF immunoassays

Cyclin-dependent kinases (CDKs) 4 & 6 play a key in breast cancer. Cyclin D1-CDK4/6 complexes are critical regulators of the cell cycle transition from the G1 to S phase. To proceed through these phases, a cell must pass a restrictive checkpoint, tightly regulated in this case by the retinoblastoma tumor suppressor protein (Rb). Palbociclib is a small molecule kinase inhibitor that blocks Cyclin D1-CDK4/6 mediated phosphorylation of the Rb protein to prevent E2F driven transcription of genes that commit the cell to DNA replication and cell division. In this application note, you will learn: The effect of palbociclib treatment in two breast cancer cell lines How to monitor the amount of phospho-Rb and Cyclin D1 protein levels with HTRF® ® immunoassays to examine the effect of CDK4/6 inhibition and Cyclin D1 regulation

Whitepaper
Whitepaper
Cell-based assays: Purposeful screens for better results

Over these last few decades there has been a growing trend in drug discovery to use cellular systems and functional assays, in addition to biochemical assays, for the characterization of new potential therapeutics. The ability to study the interaction between a candidate drug and its target within the context of a whole, intact cell allows for more physiologically relevant data to be obtained. However, such assays are more complex than traditional biochemical assays as such facts as membrane permeability, cellular metabolism, cell variability, additional binding partners, and signal transduction must be considered. To help you navigate the complexities in designing cell-based assays, we have gathered insights collected over the years and compiled them to provide you with elements to consider when setting up your cell-based assays. After all, any assay, biochemical or cell-based, is only as good as its design.

Application Note
Application Note
Characterization of candidate molecules through GTP binding assays

Enhance your GTP measurements with this application note δ-opioid receptors (DOP) have become a major target for the development of new pain treatments. This application note will show you how to characterize pharmacological compounds easily through GTP binding assays: Measuring the level of Gi protein activation Using a CHO membrane model expressing delta opioid receptor Several case studies for the different classes of pharmacological compounds

Application Note
Application Note
Characterizing compounds acting on β-arrestin2 coupled GPCRs

Dive deeper into research on the GPCR signaling pathway β-arrestins are intracellular proteins that play an important role in GPCR signaling. Complexes formed between ligand-occupied GPCRs and β-arrestin lead to interaction with adaptor protein AP2. This interaction is followed by internalization of the receptors. HTRF technology is effective for studying the interaction between AP2 and β-arrestin2. Get your application note to discover: The applicability of the β-arr2 recruitment kit to a variety of compounds acting on β-arrestin2, and its ability to correctly rank pharmacological compounds (agonists and antagonists) How you can detect the β-arrestin2 / AP2 interaction for all classes of GPCRs Detailed experimental conditions and explanatory diagrams

Whitepaper
Whitepaper
Complete gene therapy review in a single industry report

With the increased understanding of molecular and cellular medicine, more specific and efficient gene transfer vectors are now producing clinical results. 20 years after the first clinical trials, gene therapy is considered one of the greatest scientific success stories of the 21st century. While the Covid-19 crisis has disrupted the evolution in this promising field, gene therapy is expected to recover and continue its growth in the future. Download this complete industry report to learn more about the gene therapy market trends and latest advances. It includes: Gene therapies in the global pipeline and major actors in the field A description of gene therapy development and vectors used The challenges concerning gene therapy manufacturing The promising future of gene therapy

Guide
Guide
Cytokine assays: a guide to success with HTRF

The definitive guide to setting up a successful cytokine assay Many therapeutic areas require an understanding of cytokine release. When preparing for a cytokine assay, many underappreciated parameters (e.g. sample handling, cell culture format, sample dilutions) can in fact greatly impact the performance of the cytokine detection. This guide reviews the latest knowledge surrounding the proper use of HTRF cytokine assays. A review of the key terms and definitions in cytokine detection A list of optimization steps Recommendations for data analysis

Application Note
Application Note
Cytokine release from fresh blood samples

Ask real blood for real responses Fresh blood is the model of choice to study drug immunotoxicity and predict adverse effects. Written in collaboration with Blood Assay Solutions, this note provides guidelines for fresh blood cytokine quantification. Discover the power of our cytokine portfolio for your research. Features Step by step protocols for fresh blood cytokine quantification in your research Examples of pathway stimulation assays (TCR, TLR …) Comparison beteen fresh blood and PBMCs

Application Note
Application Note
Detection of MAPK activation to evaluate the efficacy and potency of KRAS/SOS1 inhibitors by AlphaLISA and HTRF technologies

Evaluation of the therapeutic profile of anti-oncogene compounds in various cell lines with AlphaLISA™ and HTRF™ KRAS is a proto-oncogene known to be mutated in many cancer subtypes, inducing uncontrolled proliferation and cell metabolism changes. Like most small GTPases, KRAS will bind to GDP in its inactive form or to GTP in its active form. KRAS G12C is one of the most commonly found mutant forms in cancers, and leads to a permanently active state of KRAS. The upregulation of KRAS interaction with the exchange factor SOS1 leads to cancer phenotypes. Reducing KRAS activity and associated pathways could control the biological processes involved in cancer growth. Furthermore, it is well known that KRAS induces activation of mitogen-activated protein kinase (MAPK), thus playing a central role in human cancers. This application note provides a convincing demonstration of the reliability of the AlphaLISA and HTRF KRAS portfolios to evaluate compound in vitro therapeutic profiles in a cellular context: Determine the effects of KRAS and SOS1 inhibitors in different human cancer cell lines Discriminate the cellular action of KRAS-targeting compounds and evaluate their effectiveness in modulating KRAS downstream pathways.

Application Note
Application Note
Determination of association and dissociation rates constants using the Tag-lite platform

Challenge the limits of binding kinetics studies This Note describes how binding kinetics studies can be enriched with a K on , K off approach by providing critical data on how the association and dissociation rates of a receptor-ligand couple can be assessed thanks to streamlined, no wash Tag-lite assays. Learn how to process and analyze the data, and discover how receptor binding kinetics offers significant insights into your compound’s mode of action. Features: Materials and methods for the experiment Data processing and result analysis Examples from our R&D