Library Prep Kits

NEXTFLEX NGS library prep kits for Illumina and Element Biosciences sequencing platforms

NGS library preparation is a critical process that involves converting raw DNA or RNA samples into a format suitable for sequencing on high-throughput platforms. This preparation includes fragmenting the nucleic acids, repairing ends, adding sequencing adapters, and enriching target regions—all essential steps to ensure accurate and comprehensive sequencing data. Our NEXTFLEX® kits simplify these steps with verified, high-quality protocols and strong technical support, making the library preparation process more efficient and reliable. Furthermore, our kits are designed for full compatibility with leading sequencing platforms such as Illumina® and Element Biosciences™, ensuring they integrate smoothly into your existing laboratory setups and workflows.

We offer a comprehensive portfolio of NEXTFLEX® Next-Generation Sequencing (NGS) library preparation kits designed to streamline your genomic workflows and deliver exceptional results. Recognizing the demands of modern research, we've ensured that our products enable seamless integration with automated liquid handling systems. This focus on automation allows for increased throughput, reduced hands-on time, and enhanced reproducibility, empowering researchers to accelerate their discoveries while maintaining robustness from the very beginning of their workflows.

We provide a diverse range of library prep kits tailored to various applications in genomics research. For DNA sequencing, our kits support whole-genome sequencing , targeted sequencing, and amplicon sequencing, facilitating the exploration of genetic variations and structural genomic changes. In transcriptomics, our RNA-seq kits are designed for mRNA sequencing, total RNA sequencing, and strand-specific RNA sequencing, enabling detailed insights into gene expression and regulation. We also offer specialized kits for small RNA and microRNA sequencing, which are vital for studying gene silencing and post-transcriptional regulation mechanisms. Complementing our core kits, we provide an extensive selection of accessory products —including Universal Blockers, NGS Cleanup Beads, ribodepletion reagents , and barcodes—that are compatible with most other library prep kits on the market including Illumina™, NEB™, Watchmaker™, Twist™, etc. This versatility allows researchers to enhance their existing workflows without the need to alter established protocols, offering cost-effective solutions that maintain flexibility and performance.

NGS adapters: Barcoded adapters for all of your NGS multiplexing needs.
Small RNA-seq library prep: Gene silencing and post-transcriptional regulation of gene expression can be investigated by small RNA-seq, which allows for the profiling of all of the small RNA and miRNA present in samples.
RNA-seq library prep: Profiling for the analysis of the expression level of RNAs in a given cell or population of cells.
DNA-seq library prep: Library prep kits for whole genome sequencing.
Targeted sequencing: Targeted resequencing for a highly targeted approach that analyses specific genomic regions.
16S rRNA sequencing: 16S amplicon panels used to identify and compare bacteria present within an environmental sample.
Single cell DNA-sequencing: Amplification of DNA from single cells and other limited DNA templates for NGS and other applications.
Reproductive genetic research: Streamlined PGT-A preparation

For research use only. Not for use in diagnostic procedures.

Request more information

Explore our solutions

Ribodepletion Solutions

Revvity offers solutions based on either CRISPR-Cas9 depletion or using a hybridization-probe approach to remove uninformative molecules prior to sequencing and improve your ability to detect biologically relevant transcripts in single cell sequencing, whole blood samples, and even complex communities containing host and bacteria RNA.

Learn more

16S rRNA Library Prep Solutions

Revvity now brings flexibility to your microbial community profiling projects with three 16S library prep kits, each covering different regions in the 16S rRNA gene. These kits are available alone or bundled with the CosmosID-Hub® analysis solution, delivering user-friendly access to version-controlled 16S pipelines.

Learn more