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NEXTFLEX NGS library prep kits for Illumina and Element Biosciences sequencing platforms

NGS library preparation is a critical process that involves converting raw DNA or RNA samples into a format suitable for sequencing on high-throughput platforms. This preparation includes fragmenting the nucleic acids, repairing ends, adding sequencing adapters, and enriching target regions—all essential steps to ensure accurate and comprehensive sequencing data. Our NEXTFLEX® kits simplify these steps with verified, high-quality protocols and strong technical support, making the library preparation process more efficient and reliable. Furthermore, our kits are designed for full compatibility with leading sequencing platforms such as Illumina® and Element Biosciences™, ensuring they integrate smoothly into your existing laboratory setups and workflows.

We offer a comprehensive portfolio of NEXTFLEX® Next-Generation Sequencing (NGS) library preparation kits designed to streamline your genomic workflows and deliver exceptional results. Recognizing the demands of modern research, we've ensured that our products enable seamless integration with automated liquid handling systems. This focus on automation allows for increased throughput, reduced hands-on time, and enhanced reproducibility, empowering researchers to accelerate their discoveries while maintaining robustness from the very beginning of their workflows.

We provide a diverse range of library prep kits tailored to various applications in genomics research. For DNA sequencing, our kits support whole-genome sequencing , targeted sequencing, and amplicon sequencing, facilitating the exploration of genetic variations and structural genomic changes. In transcriptomics, our RNA-seq kits are designed for mRNA sequencing, total RNA sequencing, and strand-specific RNA sequencing, enabling detailed insights into gene expression and regulation. We also offer specialized kits for small RNA and microRNA sequencing, which are vital for studying gene silencing and post-transcriptional regulation mechanisms. Complementing our core kits, we provide an extensive selection of accessory products —including Universal Blockers, NGS Cleanup Beads, ribodepletion reagents , and barcodes—that are compatible with most other library prep kits on the market including Illumina™, NEB™, Watchmaker™, Twist™, etc. This versatility allows researchers to enhance their existing workflows without the need to alter established protocols, offering cost-effective solutions that maintain flexibility and performance.

  • NGS adapters: Barcoded adapters for all of your NGS multiplexing needs.
  • Small RNA-seq library prep: Gene silencing and post-transcriptional regulation of gene expression can be investigated by small RNA-seq, which allows for the profiling of all of the small RNA and miRNA present in samples.
  • RNA-seq library prep: Profiling for the analysis of the expression level of RNAs in a given cell or population of cells.
  • DNA-seq library prep: Library prep kits for whole genome sequencing.
  • Targeted sequencing: Targeted resequencing for a highly targeted approach that analyses specific genomic regions.
  • 16S rRNA sequencing: 16S amplicon panels used to identify and compare bacteria present within an environmental sample.
  • Single cell DNA-sequencing: Amplification of DNA from single cells and other limited DNA templates for NGS and other applications.
  • Reproductive genetic research: Streamlined PGT-A preparation


For research use only. Not for use in diagnostic procedures.

 

Library Prep Kits

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Flyer
Flyer
A streamlined approach to genotyping with low-pass WGS

This flyer illustrates Revvity's new streamlined arroach to genotyping with low-pass NGS

Scientific Poster
Scientific Poster
A Universal Day Zero Infectious Disease Testing Strategy Leveraging CRISPR-based Sample Depletion and Metagenomic Sequencing

This poster showcases the benefits of using CRISPRclean technology for zero day infectious disease screening.

Application Note
Application Note
Automated homogenization and DNA extraction from murine GI tract tissue, cultures, and mock community for 16S sequencing

The success of sensitive downstream genomic applications such as qPCR depend on robust and reliable upstream sample preparation, which often includes proper sample/tissue homogenization and nucleic acid extraction. As the lab scales their sample throughput and their need for consistency in complex workflows, many have integrated automation-enabled protocols into their workflows. In this application note, a semi-automated workflow for nucleic acid extraction from liver tissue is presented, which leverages the Omni Bead Ruptor Elite bead mill homogenizer with the chemagic 360 nucleic acid extractor, with optional use of a JANUS automated liquid handling workstation.

Flyer
Flyer
Considerations when selecting the right barcoded adapters

Considerations when selecting the right barcoded adapters

Flyer
Flyer
CRISPR depletion enables sensitive identification of viral and bacterial populations in complex samples

This flyer showcases the benefits of using CRISPRclean technology.

Technical Note
Technical Note
CRISPRclean® depletion technology: A new tool to better understand rare diseases

Enhanced RNA-Seq delivers double the genes detected with confidence

Application Note
Application Note
CRISPRclean® Plus for SARS-CoV-2 Shotgun Metatranscriptomic Sequencing

CRISPR-based ribodepletion removes RNA “noise” from assays, allowing key insights into variants, as well as co-infections and host responses.

Datasheet
Datasheet
CRISPRclean™ Plus Stranded Total RNA Prep with rRNA Depletion (Human, Mouse, Rat, Pan Bacteria)

CRISPR-based ribodepletion strategy optimized with stranded RNA prep increases coverage of lower-expressing transcripts from complex biological samples.

Datasheet
Datasheet
CRISPRclean™ Stranded Total RNA Prep with rRNA Depletion (Human, Mouse, Rat)

CRISPR-based ribodepletion strategy improves sensitivity for the detection of lower expressing transcripts from mammalian tissues

Brochure
Brochure
Decoding library complexity

This document provides guidance on the applications in which NEXTFLEX UDI-UMI Barcodes can be used.

Technical Note
Technical Note
DepleteX™ Globin Depletion Kit

Remove contaminating globin fragments for RNA-Seq

Technical Note
Technical Note
DepleteX™ Single Cell RNA Boost Kit

Remove abundant and uninformative fragments prior to scRNA-Seq

Application Note
Application Note
DOPlify v2 with PicoPLEX App Note

In this appplication note the DOPlify® v2 WGA kit and Takara® PicoPLEX® Single Cell WGA kit were compared and performed comparably when analyzed for whole chromosome copy number variations and targeted panel coverage when using 5-cell samples as input.

Application Note
Application Note
DOPlify WGA kit App Note

The DOPlify® kit from Revvity is a whole genome amplification kit designed and optimized specifically to amplify the genome from single cells and limited DNA templates.

Flyer
Flyer
Efficient DNA purification using SPRI technology

This flyer describes showcases the NEXTFLEX NGS Cleanup Bead specifications and uses cases.

Infographic
Infographic
Elevate your single-cell sequencing: Beat the stats

Discover simple strategies to overcome single cell sequencing obstacles in our infographic.

Flyer
Flyer
End-to-end metagenomics solutions.

This flyer showcases Revvity's metagenomic portfolio as well as CosmosID's award winning analysis solution.

Flyer
Flyer
End-to-end whole genome sequencing metagenomics solutions

This flyer describes Revvity and CosmosIDs solution for WGS for metagenomics.

Product Info
Product Info
FASTQ file containing control data for the DepleteX Single Cell RNA Boost Kit

Please fill out the form below to access a FASTQ file containing control data for the DepleteX™ Single Cell RNA Boost Kit.

Product Information Bulletin
Product Information Bulletin
FASTQ file containing depletion data for the DepleteX™ Single Cell RNA Boost Kit

Please fill out the form below to access a FASTQ file containing depletion data for the DepleteX™ Single Cell RNA Boost Kit.

Flyer
Flyer
Gel-free small RNA library prep kit for Illumina sequencing

This flyer describes the benefits of the NEXTFLEX® Small RNA-Seq Kit v4 kit

Flyer
Flyer
Get the answers you need with single-cell sequencing

Flyer showcasing Revvity's solutions for key steps of your single-cell sequencing journey.

Application Note
Application Note
High-throughput, end-to-end Cell Free DNA (cfDNA) analysis workflow from plasma

Benefit of an automated cfDNA analysis workflow The presence of double stranded, circulating cell free DNA (cfDNA) in blood plasma was discovered more than 70 years ago. Soon after, a link between cfDNA and leukemia as well as autoimmune disease was found. Recently, the development of cfDNA-based prenatal genetic testing and the realization that cfDNA can be used to detect and monitor tumor specific mutations in cancer patients has increased the interest in cfDNA analyses. Read the application note to review the results and how much time you could save by automating your cfDNA analysis workflow using Revvity’s automated solution including blood fractionation, cfDNA isolation, library prep, QC, and quantitation.

Technical Note
Technical Note
Identify more genes per UMI with CRISPRclean® and Spatial Gene Expression

Boost informative transcripts and detect low expression genes in fresh frozen tissues

Application Note
Application Note
Increase bacterial species detection in microbiome stool samples

Performance comparison study of CRISPRclean® Plus and Illumina® Ribo-Zero™ Plus for microbiome analysis