Western Lightning ONE Pico, Chemiluminescent Substrate (500 mL)

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Western Lightning ONE Pico, Chemiluminescent Substrate (500 mL)

Our one-component chemiluminescent horseradish peroxidase (HRP) substrates are ideal for routine analysis detection of abundant proteins. Western Lightning™ ONE Pico can detect mid-picogram protein levels in western blots and provides low background for a high signal to noise ratio.

For research use only. Not for use in diagnostic procedures.

Product information

SDS, COAs, Manuals and more

Product Variants

partnumber

Part number: NEL130001EA

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Unit Size: 25 mL

List price: USD 84.79

Your price:

USD 0.00

USD 84.79 /each

partnumber

Part number: NEL131001EA

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Unit Size: 250 mL

List price: USD 278.15

Your price:

USD 0.00

USD 278.15 /each

partnumber

Part number: NEL132001EA

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Unit Size: 2 x 250 mL

List price: USD 460.13

Your price:

USD 460.13

USD 460.13 /each

Product information

Overview

Part of our premier line of premixed chemiluminescent HRP substrates, Western Lightning ONE Pico offers mid-picogram detection with no mixing required. Designed for sensitive and consistent detection of proteins, this single reagent format enables greater experimental consistency while remaining an economical choice for your everyday detection needs. Stored and ready-to-use at room temperature, the 12 month extended shelf life means you can buy and use Western Lightning ONE Pico with confidence. Unit size 2 x 250 mL.

Comparison of Western Lightning^™ ONE Versus Major Competitors

Performance and Signal Duration

Western blotting detection of HDAC-1 in HeLa cell lysate
Exposure time is 120 secs for each time point
Images captured with ImageQuant^™ LAS 4000 from GE Healthcare

Additional product information

Performance	Signal Duration
Detection Level: Mid-picogram

Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane Primary antibody:Rabbit-anti Human HDAC-1 1:2000 Secondary antibody:Goat anti-rabbit IgG HRP (2mg/mL) 1:20000
Detection Level: Mid-femtogram

Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane Primary antibody: Rabbit-anti Human HDAC-1 1:5000 Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:75000
Detection Level: Low-femtogram

Two-fold dilution series of HeLa whole cell lysate from 2.5 μg to 0.039 μg of total protein on nitrocellulose membrane Primary antibody: Rabbit-anti Human HDAC-1 1:10000 Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:200000

Specifications

Other Specifications

Application	Western blotting
Brand	Western Lightning ONE
Detection Method	Chemiluminescence
Sample Type	Chemiluminescent Substrate
Shipping Conditions	Shipped Ambient
Unit Size	2 x 250 mL