Skip to main content
Menu
US

Western Lightning ONE Femto Ultra, Chemiluminescent Substrate (250 mL)

When proteins are low and antibodies are precious, our extreme-level pre-mixed one-component chemiluminescent horseradish peroxidase (HRP) substrate with low antibody consumption is the right choice. Western Lightning™ ONE Femto Ultra can detect low-femtogram protein levels in western blots with superior signal intensity.

For research use only. Not for use in diagnostic procedures.

When proteins are low and antibodies are precious, our extreme-level pre-mixed one-component chemiluminescent horseradish peroxidase (HRP) substrate with low antibody consumption is the right choice. Western Lightning™ ONE Femto Ultra can detect low-femtogram protein levels in western blots with superior signal intensity.

For research use only. Not for use in diagnostic procedures.

Product Variants
Unit Size: 25 mL
Part #:
NEL150001EA
List Price
USD 134.42
Unit Size: 125 mL
Part #:
NEL151001EA
List Price
USD 433.25
Unit Size: 2 x 125 mL
Part #:
NEL152001EA
List Price
USD 747.58

Overview

Our most sensitive substrate in our premier line of premixed chemiluminescent HRP substrates, Western Lightning ONE Femto Ultra offers low-femtogram detection with excellent signal intensity. Western Lightning ONE Femto Ultra is designed with maximum sensitivity in a single reagent format. Stored and ready-to-use at room temperature, the 12 month extended shelf life means you can buy and use Western Lightning ONE Femto Ultra with confidence. Unit size 2 x 125 mL.

Comparison of Western Lightning™ ONE Versus Major Competitors

Performance and Signal Duration

  • Western blotting detection of HDAC-1 in HeLa cell lysate
  • Exposure time is 120 secs for each time point
  • Images captured with ImageQuant™ LAS 4000 from GE Healthcare

Additional product information

Performance Signal Duration

Detection Level: Mid-picogram

wlo-pico-1
wlo-pico-web
  • Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane
  • Primary antibody:Rabbit-anti Human HDAC-1 1:2000
  • Secondary antibody:Goat anti-rabbit IgG HRP (2mg/mL) 1:20000

Detection Level: Mid-femtogram

wlo-femto-web
wlo-femto-web
  • Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane
  • Primary antibody: Rabbit-anti Human HDAC-1 1:5000
  • Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:75000

Detection Level: Low-femtogram

wlo-femto-ultra-web
wlo-femto-ultra-web
  • Two-fold dilution series of HeLa whole cell lysate from 2.5 μg to 0.039 μg of total protein on nitrocellulose membrane
  • Primary antibody:  Rabbit-anti Human HDAC-1 1:10000
  • Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:200000

 

Specifications

Application
Western blotting
Brand
Western Lightning ONE
Detection Modality
Chemiluminescence
Sample Type
Chemiluminescent Substrate
Shipping Conditions
Shipped Ambient
Unit Size
2 x 125 mL

Video gallery

Resources

Are you looking for resources, click on the resource type to explore further.

1-3 of 3 Resources
Flyer Icon
Flyer
The power of Western Lightning ONE HRP chemiluminescent substrates

One-Reagent Substrates for Western Blotting!

Get the consistency you demand from your western blot experiments with pre-mixed, one...

Application Note Icon
Application Note
Western blot validation of CRISPR-Cas9-induced functional knockout using synthetic sgRNA molecules

Any well-designed CRISPR experiment must include a method to assess the accuracy and reliability of predicted gene editing results...

Brochure Icon
Brochure
Western blotting solutions

With applications in biotechnology, molecular biology, proteomics, and more, western blotting is the most widely used technique...

Scroll Icon