Western Lightning ONE Femto, Chemiluminescent Substrate (250 mL)

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Western Lightning ONE Femto, Chemiluminescent Substrate (250 mL)

A versatile, mid-level pre-mixed, one-component chemiluminescent horseradish peroxidase (HRP) substrate appropriate for a wide range of antibody dilutions, Western Lightning^™ ONE Femto can detect mid-femtogram protein levels in western blots with a high signal intensity.

For research use only. Not for use in diagnostic procedures.

Product information

SDS, COAs, Manuals and more

Product Variants

partnumber

Part number: NEL140001EA

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Unit Size: 25 mL

List price: USD 79.62

Your price:

USD 0.00

USD 79.62 /each

partnumber

Part number: NEL141001EA

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Unit Size: 250 mL

List price: USD 281.25

Your price:

USD 281.25

USD 281.25 /each

partnumber

Part number: NEL142001EA

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Unit Size: 2 x 250 mL

List price: USD 443.59

Your price:

USD 0.00

USD 443.59 /each

Product information

Overview

Get accurate detection of both low and high abundance proteins in the same experient. Part of our premier line of premixed chemiluminescnet HRP substrates, Western Lightning ONE Femto offers the flexibility of mid-femtogram detection with excellent dynamic range. This substrate offers sensitive and consistent detection of proteins with no mixing or measuring parts required. Stored and ready-to-use at room temperature, the 12 month extended shelf life means you can buy and use Western Lightning ONE Femto with confidence. Available in 250 mL size.

Comparison of Western Lightning^™ ONE Versus Major Competitors

Performance and Signal Duration

Western blotting detection of HDAC-1 in HeLa cell lysate
Exposure time is 120 secs for each time point
Images captured with ImageQuant^™ LAS 4000 from GE Healthcare

Additional product information

Performance	Signal Duration
Detection Level: Mid-picogram

Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane Primary antibody:Rabbit-anti Human HDAC-1 1:2000 Secondary antibody:Goat anti-rabbit IgG HRP (2mg/mL) 1:20000
Detection Level: Mid-femtogram

Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane Primary antibody: Rabbit-anti Human HDAC-1 1:5000 Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:75000
Detection Level: Low-femtogram

Two-fold dilution series of HeLa whole cell lysate from 2.5 μg to 0.039 μg of total protein on nitrocellulose membrane Primary antibody: Rabbit-anti Human HDAC-1 1:10000 Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:200000

Specifications

Other Specifications

Application	Western blotting
Brand	Western Lightning ONE
Detection Method	Chemiluminescence
Sample Type	Chemiluminescent Substrate
Shipping Conditions	Shipped Ambient
Unit Size	250 mL