The Operetta CLS system combines speed and sensitivity with the powerful and intuitive data analysis you’ve come to trust from the Operetta platform. The all-new Operetta CLS delivers everything you need from the high-content analysis. What’s more, the Operetta CLS system is part of our comprehensive HCS workflow – everything from HCS systems and microplates to automation and informatics for every application. All from one knowledgeable, trusted vendor. Put that together with our Harmony™
high-content imaging and analysis software – the easy-to-learn, easy-to-use software that empowers biologists to do their own analysis – and you have everything you need to run your every day (and complex) analyses right away.

Applications

From everyday assays to more demanding applications, the Operetta CLS high-content analysis system delivers just the right combination of flexible excitation, sensitive optics, and advanced software features to enable you to gain deeper biological insight from all your critical applications.

Fixed-cell assays

• Up to eight high-power excitation sources and user-accessible emission filters enable maximum flexibility of fluorescent stains and labels, plus the system features widefield and spinning-disk fluorescent imaging.

Live-cell assays

• The spinning disk confocal optics and synchronized LED illumination provides stable excitation and minimize phototoxicity and bleaching for meaningful live-cell assays. You can also choose brightfield or digital-phase contrast imaging modes.

Complex cellular models

• The large-format sCMOS camera with water-immersion objectives provides sensitivity and high resolution, while advanced software helps you address the imaging and analysis challenges presented by complex cellular models.

Advanced assays

• FRET is a powerful tool for investigating conformational changes and protein-protein interactions. The Operetta CLS system’s sensitive imaging and dedicated analysis tools for ratiometric imaging, facilitate robust results.

Phenotypic fingerprinting

• The Operetta CLS combines high-resolution imaging with advanced software tools to help create robust phenotypic fingerprints of the subtle differences at the core of successful phenotypic assays.

Your complete HCS workflow

• Get superior results with our PhenoPlate™ microplates for high content screening
• Use together with our PhenoVue™ cellular imaging reagents including cell painting kits
• Improve throughput and productivity by automating your Operetta CLS system, or benefit from our automated cellular workflows and drug discovery workflows
• Export results automatically into Signals Image Artist™ Image Analysis and Management platform, so you can access, re-analyze, store, and share all your cell image data from Operetta CLS and other HCS systems
• Combine with Signals VitroVivo for powerful multivariate statistical methods and unsupervised machine learning techniques so you can identify parameters that best define distinct cellular fingerprints