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Technical Note
The benefits of water immersion lenses for high-content screening
The key parameters in high-content imaging - speed, sensitivity (or intensity) and resolution - cannot be optimized independently as altering one of them influences the others. For example, increasing the resolution by choosing a higher magnification requires imaging of more fields to maintain the same cell number – at the expense of speed; or increasing the fluorescence intensity can be achieved by increasing the exposure time – again at the expense of speed. Nevertheless, there is a way to overcome some of these obstacles and here, in this Technical Note, we explain why the choice of the objective lens is critical.
For research use only. Not for use in diagnostic procedures.
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The benefits of water immersion lenses for high-content screening