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HTRF Human Apolipoprotein C3 Detection Kit, 500 Assay Points
The ApoC3 kit is designed for the simple quantification of ApoC3 in cell-based formats.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Product information
Overview
Apolipoproteins are proteins that bind to lipids to form lipoproteins, whose main function is to transport lipids. Apolipoproteins are important in maintaining the structural integrity and solubility of lipoproteins, and play an important role in lipoprotein receptor recognition and regulation of certain enzymes in lipoprotein metabolism. ApoC3 inhibits lipoprotein lipase and hepatic lipase. Increased ApoC3 expression may lead to hypertriglyceridaemia and an atherogenic lipoprotein profile.
Specifications
| Assay Points |
500
|
|---|---|
| Assay Target Type |
Kit
|
| Assay Technology |
HTRF
|
| Brand |
HTRF
|
| Quantity |
1
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Therapeutic Area |
Cardiovascular
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
|
| Unit Size |
500 Assay Points
|
Video gallery
How it works
Assay principle
The Apolipoprotein C3 assay is a sandwich immunoassay involving two monoclonal antibodies, one labelled with Eu-Cryptate (Donor) and the second with d2 (Acceptor). The intensity of the signal is proportional to the concentration of apolipoprotein present in the sample.
Assay protocol
The ApoC3 assay features a streamlined protocol with only one incubation step following the sample/calibrator and Antibody conjugates dispensing. The antibodies labelled with HTRF donor and acceptor may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. This protocol requires a single 2-hour incubation period at RT.
Assay details
| Human Apo CIII | |
|---|---|
| Incubation | 2h at RT (or ON) |
| Assay range | 0.321 to 125 ng/mL |
| Detection limit | 0.112 ng/mL |
Analytical performance
Intra/Inter assay
Intra-assay (n=22)
| Sample | Mean [ApoC3] (ng/mL) | CV |
|---|---|---|
| 1 | 1.15 | 13.2 |
| 2 | 11.8 | 9 |
| 3 | 121.8 | 9.5 |
| Mean CV | 10.5 |
Each of the 3 samples was measured 22 times, and the %CV for the concentration was calculated for each sample.
Inter-assay (n=4)
| Sample | [ApoC3] (ng/mL) | Mean (delta R) | CV |
|---|---|---|---|
| 1 | 9.79 | 2986 | 8 |
| 2 | 50.6 | 12644 | 11 |
| 3 | 105 | 21141 | 7 |
| Mean CV | 8.6 |
Each of the samples was measured in 4 different experiments, and the %CV for the concentration was calculated for each sample.
Resources
Whitepaper
An overview of atherosclerosis
Atherosclerosis pathogenesis, cellular actors, and pathways
Atherosclerosis is a common condition in which arteries harden and...
Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
SDS, COAs, Manuals and more
Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.
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