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HTRF GST check Detection Kit, 500 Assay Points

The GST check kit enables the rapid detection of GST tagged fusion proteins.

View product information

Feature	Specification
Application	Protein-Protein Interaction
Sample Volume	10 µL

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The GST check kit enables the rapid detection of GST tagged fusion proteins.

View product information

Product Variant

Part #:

62GSTPEG

List Price

USD 1,082.64

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Product information

Overview

The kit enables the rapid detection of GST tagged fusion proteins at each step of protein production and purification. It can either be used to ensure that the GST motif of a fusion protein is accessible to the anti-GST antibody, or to approximate the concentration of a GST-tagged protein. Calibrated using the appropriate protein, the assay is very accurate and compatible with the SOPs. It can also be used to eliminate false positives.

Specifications

Other Specifications

Application	Protein-Protein Interaction
Brand	HTRF
Detection Modality	HTRF
Product Group	Kit
Sample Volume	10 µL
Shipping Conditions	Shipped Ambient
Target Class	Binding Assay
Technology	TR-FRET
Therapeutic Area	Cardiovascular Infectious Diseases Inflammation Metabolism/Diabetes NASH/Fibrosis Neuroscience Oncology & Inflammation Rare Diseases
Unit Size	500 Assay Points

Video gallery

How it works

Assay principle

As shown in the graph, GST peptide labeled with XL665 is detected by anti GST Cryptate conjugate (anti-tag Cryptate). The GST-tagged fusion protein (Tag fusion protein) to be ascertained competes with the GST-peptide-XL665 conjugate (Tag-XL665) for binding to the anti-GST-Cryptate conjugate. The specific signal is inversely proportional to the concentration of GST-tagged fusion protein.

Assay protocol

The example on the right describes the protocol using a 20 µL final assay volume. Dispense 10 µL of standard or of GST-tagged protein of interest into a well, with 5 µL of GST Cryptate conjugate and 5 µL of GST-XL665 conjugate. Incubate for 2 hours at RT and read.

Assay validation

Native GST standard curve using GST Check Kit

Detection limit:	1 ng/mL (average)
S/B	15 in 96- or 384-well formats

GST Check Kit performance on various GST-tagged proteins

The GST check kit enables the verification of tag accessibility on GST-tagged fusion proteins. The figure shows the dose response curves of different GST fusion proteins assessed using this kit. All six GST containing proteins were detected using our competitive immunoassay.