Kinetic values are of importance for characterizing protein-protein interactions. Studying them brings insight regarding macromolecule binding. Accessing kinetic values is therefore important.
This application note dives deep into the topic to provide key guideline and experiments for determining true Kd values in protein-protein interaction assays with HTRF.
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For research use only. Not for use in diagnostic procedures.
How to access true Kd values in Protein-Protein Interaction assays