Reporter Gene Luminescence Assays
Intensity. Flexibility. Just right for every assay
Our reporter gene assay reagents produce a long-lived glow for plate batch processing and simplified, automated, cost-effective screens. Our luciferase assay reagent options have a half-life of several hours, allowing for streamlined detection procedures.
Use our reporter assays to characterize the strength of promoters and enhancers, define the role of transcription factors, or assess transfection efficiency during drug development.
Benefits include:
- Convenient one-step processing: No washes required – simply mix, incubate, and read
- Strong signal for remarkably accurate and thorough firefly or renilla luciferase reading
- Enhanced stability: All our luciferase reporter assay options are designed for extended storage at 2-8°C
- No DTT: Unlike most luciferase detection reagents, our reporter gene assay options do not contain DTT, eliminating toxic hazards and the need for hood work
- Amenable to high throughput and ultra-high throughput environments, using 384- or 1536-well microplate formats
- Excellent Z' Values: Each luciferase assay system offers robust, sensitive performance, with high signal-to-background ratios
Our luciferase reporter assay options include:
- britelite™ plus: ideal for assays requiring the utmost sensitivity
- neolite™: this reporter assay offers the ultimate compromise between strong signal intensity and extended signal half-life
- steadylite™ plus: the reagent of choice for HTS where you require the same steady signal first plate into last plate out
- sensilite™: a reporter assay designed to provide maximum signal intensity
- twinlite™: ideal reporter assay for normalization for high quality data and to screen two events in parallel
Whether your reporter assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, Revvity offers a reporter assay to suit your needs.
For research use only. Not for use in diagnostic procedures.
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twinlite is a dual luciferase reporter gene assay system designed to detect and quantitate both Firefly and Renilla luciferase sequentially from cultured cells. It requires the use of a luminometer equipped with two dispensers as the signal has to be read shortly after dispensing.
Our brightest reporter gene assay for quantitation of firefly luciferase in mammalian cells, britelite™ Plus offers very high sensitivity, is designed for continuous process systems and higher density microplates.
twinlite™ glow is a dual luciferase reporter gene assay system designed to detect and quantitate both Firefly and Renilla luciferase sequentially from cultured cells.
Sensilite is a firefly luciferase assay combining benefits of both bright signal and long-lasting signal for firefly luciferase.
Steadylite is a reporter gene assay with the longest-lasting signal for quantitation of firefly luciferase in mammalian cells.
The Passive Lysis Solution (PLS) is a mammalian cell lysis solution intended to be used to prepare cell lysates for measurement of luciferase activity with the twinlite and sensilite reporter gene assay systems. It is provided in the twinlite and sensilite kits, but if additional volume of the lysis solution is required, it is also available as a separate item, 100 mL volume per bottle.