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Radiometric

Solid Scintillant Alternative - MeltiLex

Section
Liquid Scintillation Counting
Thymidine Uptake Assays
DNA and RNA Labeling
Scintillation Proximity Assays
In Vitro Kinase Assays
CAT Reporter Gene Assays
Cell and Metabolic Labeling
Chromium-51 Release Assay
Sulfur-35 Methionine Labeling
Iodine-125 Labeling of Proteins
Sulfur-35 GTP Binding Assays
Radiochemical Calculations
Glucose Uptake Assays
Radiometric Assays and Detection
Radiometric Ligand-Binding Assays
Phosphorylation Assays
Acetylation Assays Using Acetyl CoA
Radioimmunoassays
Phosphorus-32 Cell Labeling Assays
Liquid Scintillation Counting
Which Formulation Should I Choose?
Sub Section
Solid Scintillant Alternative - MeltiLex
Solid Scintillant Alternative - MeltiLex
Liquid Scintillation Cocktails
Alpha-Beta Discrimination
Sample Preparation for LSC
Quench, Counting Efficiency, and Quench Correction

Overview

MeltiLex™ is a solid scintillant alternative to liquid scintillation cocktails for Filtermat samples. MeltiLex is a unique melt-on scintillator for dry on support counting for use with Filtermat.

  • MeltiLex A – used with Filtermat A (catalog number 1450-421). GF/C filters are thin, and typically used for whole cell assays such as 3H-thymidine incorporation assays.
  • MeltiLex B – used with Filtermat B (catalog number 1450-521). GF/B filters are typically used in cell-membrane based assays, such as receptor-ligand binding assays.

The Filtermat -plus-cocktail combination provides increased tritium counting efficiency. In a thymidine uptake experiment, the relative counting efficiencies of Filtermat with MeltiLex and HARVEST Plate with cocktail are 53% and 68%, respectively. Both methods exhibited similar consistencies in count rate and therefore harvesting efficacy. Results from the two-dilution series also showed excellent correlation.

For research use only. Not for use in diagnostic procedures

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