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Application Support Knowledge Base

Application Support Knowledge Base

The application support knowledge base (ASK) provides a comprehensive set of technology-specific and application-specific scientific articles, written by our R&D, technical support, and applications teams for scientists, enabling you to access scientific support 24 hours a day/7 days a week, with content-rich information covering assay design and assay development, product selection, detailed sample protocols, sample data, data analysis, tips, troubleshooting tables, video tutorials, and selected citations for common assays developed and run using Revvity technologies.

AlphaLISA
AlphaLISA™ is a bead-based assay technology used to study biomolecular interactions in a microplate format. The acronym "Alpha" stands for amplified luminescent proximity homogeneous assay.

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Alpha Antibody Detection and Characterization
Alpha Bead Conjugation
Alpha Citations
Alpha EMSA Conversion
Alpha Immunogenicity
Alpha Instrument Options and Settings
Alpha Products and Catalog Numbers
Alpha Protein-protein and Protein-nucleic Acid Interactions
Alpha Troubleshooting Tables
AlphaLISA 5 μL vs. 20 μL Sample Volume
AlphaLISA Epigenetic Toolbox Reagents
AlphaLISA Immunoassay Kits
AlphaLISA and AlphaScreen No-wash Assays
Bead Selection and Bead Interference
Buffer Selection for Alpha Assays
Create your own Alpha Assay
Data Analysis for AlphaLISA Immunoassays
Determining Kd With an Alpha Assay
Other Alpha Applications
Preparation of Analyte-depleted Serum for Alpha Assays
The Hook Effect
Working with Serum and Other Biological Matrices in Alpha Assays
Working with cell extracts and supernatants in Alpha assays

AlphaLISA SureFire Ultra
AlphaLISA SureFire Ultra assay kits allow the rapid, sensitive, and quantitative detection of phospho- and total proteins from cells. The AlphaLISA SureFire Ultra assay kits are optimized for enhanced signal-to-noise windows.
DELFIA
DELFIA™ (dissociation-enhanced lanthanide fluorescence immunoassay) is a time-resolved fluorescence (TRF) intensity technology. Assays are designed to detect the presence of a compound or biomolecule using lanthanide chelate labeled reagents, separating unbound reagent using wash steps.
In Vivo Preclinical Imaging
Pre-clinical imaging techniques including  in vivo, ex vivo, and in vitro imaging enable the understanding of disease from cellular to whole animal models and are used in the translation to, and development of therapies in clinical-relevant models.
LANCE
ULight™ dye absorption (excitation) and emission spectra.
Microplates
Revvity offers a range of 96-well, 384-well and 1536-well tissue culture treated imaging plates. These include the following:
Radiometric
NEN radiochemicals are available in a variety of concentrations, specific activities, buffers, shipping, packaging and storage conditions.
Cell Counting and Image Cytometry
Nexcelom’s leading automated cell counting and image cytometry solutions are now part of Revvity’s portfolio.The portfolio includes the Celigo™ image cytometer, the Cellaca™ MX high-throughput cell counter, and Cellometer™ automated cell counters. Here you can find information to support your use of these instruments together with our comprehensive range of complementary consumables and reagents.
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