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Application Support Knowledge Base

Application Support Knowledge Base

The application support knowledge base (ASK) provides a comprehensive set of technology-specific and application-specific scientific articles, written by our R&D, technical support, and applications teams for scientists, enabling you to access scientific support 24 hours a day/7 days a week, with content-rich information covering assay design and assay development, product selection, detailed sample protocols, sample data, data analysis, tips, troubleshooting tables, video tutorials, and selected citations for common assays developed and run using Revvity technologies.

AlphaLISA
AlphaLISA™ is a bead-based assay technology used to study biomolecular interactions in a microplate format. The acronym "Alpha" stands for amplified luminescent proximity homogeneous assay.
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Alpha Antibody Detection and Characterization
Alpha Bead Conjugation
Alpha Citations
Alpha EMSA Conversion
Alpha Immunogenicity
Alpha Instrument Options and Settings
Alpha Products and Catalog Numbers
Alpha Protein-protein and Protein-nucleic Acid Interactions
Alpha Troubleshooting Tables
AlphaLISA 5 μL vs. 20 μL Sample Volume
AlphaLISA Epigenetic Toolbox Reagents
AlphaLISA Immunoassay Kits
AlphaLISA and AlphaScreen No-wash Assays
Bead Selection and Bead Interference
Buffer Selection for Alpha Assays
Create your own Alpha Assay
Data Analysis for AlphaLISA Immunoassays
Determining Kd With an Alpha Assay
Other Alpha Applications
Preparation of Analyte-depleted Serum for Alpha Assays
The Hook Effect
Working with Serum and Other Biological Matrices in Alpha Assays
Working with cell extracts and supernatants in Alpha assays
AlphaLISA SureFire Ultra
AlphaLISA SureFire Ultra assay kits allow the rapid, sensitive, and quantitative detection of phospho- and total proteins from cells. The AlphaLISA SureFire Ultra assay kits are optimized for enhanced signal-to-noise windows.
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Alpha SureFire No-wash Cellular Kinase Assays
FAQs and Guidelines for Successful AlphaLISA SureFire Assay Setup
DELFIA
DELFIA™ (dissociation-enhanced lanthanide fluorescence immunoassay) is a time-resolved fluorescence (TRF) intensity technology. Assays are designed to detect the presence of a compound or biomolecule using lanthanide chelate labeled reagents, separating unbound reagent using wash steps.
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DELFIA Biodistribution
DELFIA Buffers
DELFIA Cell Cytotoxicity Assays
DELFIA Cell Proliferation Assay
DELFIA Citations
DELFIA Immunoassays
DELFIA Instrument Options and Settings
DELFIA Kinase Assays
DELFIA Receptor-Ligand Binding Assays
DELFIA Time-Resolved Fluorescence Assays
DELFIA products and catalog numbers
Label your own DELFIA reagents
Microplates For Absorbance and Colorimetric Assays
Multiplexing DELFIA™ assays
In Vivo Preclinical Imaging
Pre-clinical imaging techniques including  in vivo, ex vivo, and in vitro imaging enable the understanding of disease from cellular to whole animal models and are used in the translation to, and development of therapies in clinical-relevant models.
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Applications
Best Practices
IVISbrite Bacterial Cell Lines
IVISbrite Bioluminescent Substrates
IVISbrite Lentiviral Particles
IVISbrite Tumor Cell Lines
IVISense Fluorescent Dyes
IVISense Fluorescent Imaging Probes - Activatable
IVISense Fluorescent Imaging Probes - Targeted
IVISense Fluorescent Imaging Probes - Vascular
In Vivo Imaging
LANCE
ULight™ dye absorption (excitation) and emission spectra.
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APC dye spectra
LANCE Citations
LANCE Classic 3- Kinase Assay
LANCE Epigenetic Assays
LANCE Eu-W1024-ITC spectra
LANCE Eu-W8044-DTA spectra
LANCE Indirect 4-Component Kinase Assays
LANCE Instrument Options and Settings
LANCE Phosphatase Assays
LANCE Products and Catalog Numbers
LANCE Protein-Protein Interaction Assays
LANCE ULight dye spectra
LANCE Ultra Kinase Assays
LANCE Ultra cAMP Assays
LANCE and LANCE Ultra TR-FRET Assays
LANCE kinase assays optimization
Label Your Own LANCE Reagent
Suppliers for other cAMP reagents
Suppliers for other kinase assay reagents
Microplates
Revvity offers a range of 96-well, 384-well and 1536-well tissue culture treated imaging plates. These include the following:
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Microplate Dimensions, Working Volumes and Packaging
Microplates For Fluorescence Assays
Microplates For High Content Analysis and High Content Screening Assays
Microplates For Luminescence Assays
Microplates Knowledge Base
Microplates and Consumables for Next Generation Sequencing
Microplates for High Content Analysis and High Content Screening Assays
Microplates for Radiometric Assays
Microplates for Storage
Plate Treatments, Coatings and Applications
Table of Microplates by Application
Radiometric
NEN radiochemicals are available in a variety of concentrations, specific activities, buffers, shipping, packaging and storage conditions.
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Acetylation Assays Using Acetyl CoA
CAT Reporter Gene Assays
Cell and Metabolic Labeling
Chromium-51 Release Assay
DNA and RNA Labeling
Glucose Uptake Assays
In Vitro Kinase Assays
Iodine-125 Labeling of Proteins
Liquid Scintillation Counting
Phosphorus-32 Cell Labeling Assays
Phosphorylation Assays
Radiochemical Calculations
Radioimmunoassays
Radiometric Assays and Detection
Radiometric Ligand-Binding Assays
Scintillation Proximity Assays
Sulfur-35 GTP Binding Assays
Sulfur-35 Methionine Labeling
Thymidine Uptake Assays
Which Formulation Should I Choose?
Cell Counting and Image Cytometry
Nexcelom’s leading automated cell counting and image cytometry solutions are now part of Revvity’s portfolio.The portfolio includes the Celigo™ image cytometer, the Cellaca™ MX high-throughput cell counter, and Cellometer™ automated cell counters. Here you can find information to support your use of these instruments together with our comprehensive range of complementary consumables and reagents.
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