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SureLight Allophycocyanin-anti-6His Antibody, 1 mg

APC-labeled anti-His antibody, for capturing His-tagged proteins and peptides in LANCE TR-FRET assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Part number: AD0059H
List price: USD 810.88
Your price:
USD 810.88
USD 810.88 /each

Overview

Mouse monoclonal antibody against 6xHis polypeptide conjugated to allophycocyanin (APC). APC is a fluorescent light harvesting protein unique to cyanobacteria and red algae and a member of the phycobiliprotein family of direct fluorescent dyes. Surelight APC is a chemically and intramolecularly cross-linked form of a protein. It is produced using a proprietary manufacturing process to increase thermal stability while maintaining the fluorescent and spectral properties of native dye.

LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).

Specifications

Antibody Conjugates
Anti-6X His
Application
Protein-protein interactions
Automation Compatible
Yes
Brand
LANCE SureLight
Detection Method
Time-Resolved Fluorescence (TRF)
TR-FRET
Experimental Type
In vitro
Fluorophore
SureLight APC
Format
Microplates
One Unit Contains
1.0 mg
Unit Size
1 mg

Citations

Resources

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Technical Note
How to access true Kd values in Protein-Protein Interaction assays

Investigate the dynamics of Interactions

Kinetic values are of importance for characterizing protein-protein interactions...

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