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SureLight Allophycocyanin-anti-6His Antibody, 1 mg

APC-labeled anti-His antibody, for capturing His-tagged proteins and peptides in LANCE TR-FRET assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Feature Specification
Application Protein-Protein Interaction

APC-labeled anti-His antibody, for capturing His-tagged proteins and peptides in LANCE TR-FRET assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Product Variant
Unit Size: 1 mg
Part #:
AD0059H
List Price
USD 810.88

Overview

Mouse monoclonal antibody against 6xHis polypeptide conjugated to allophycocyanin (APC). APC is a fluorescent light harvesting protein unique to cyanobacteria and red algae and a member of the phycobiliprotein family of direct fluorescent dyes. Surelight APC is a chemically and intramolecularly cross-linked form of a protein. It is produced using a proprietary manufacturing process to increase thermal stability while maintaining the fluorescent and spectral properties of native dye.

LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).

Specifications

Application
Protein-Protein Interaction
Automation Compatible
Yes
Brand
LANCE SureLight
Conjugates
Anti-6X His
Detection Modality
LANCE
Fluorophore
SureLight APC
Product Group
Antibody
Shipping Conditions
Shipped in Blue Ice
Target Class
Binding Assay
Technology
TR-FRET
Unit Size
1 mg

Citations

Resources

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Application Note
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Investigate the dynamics of Interactions

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Product Info
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