APC-labeled anti-His antibody, for capturing His-tagged proteins and peptides in LANCE TR-FRET assays.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Feature | Specification |
---|---|
Application | Protein-Protein Interaction |
APC-labeled anti-His antibody, for capturing His-tagged proteins and peptides in LANCE TR-FRET assays.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Mouse monoclonal antibody against 6xHis polypeptide conjugated to allophycocyanin (APC). APC is a fluorescent light harvesting protein unique to cyanobacteria and red algae and a member of the phycobiliprotein family of direct fluorescent dyes. Surelight APC is a chemically and intramolecularly cross-linked form of a protein. It is produced using a proprietary manufacturing process to increase thermal stability while maintaining the fluorescent and spectral properties of native dye.
LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).
Application |
Protein-Protein Interaction
|
---|---|
Automation Compatible |
Yes
|
Brand |
LANCE SureLight
|
Conjugates |
Anti-6X His
|
Detection Modality |
LANCE
|
Fluorophore |
SureLight APC
|
Product Group |
Antibody
|
Shipping Conditions |
Shipped in Blue Ice
|
Target Class |
Binding Assay
|
Technology |
TR-FRET
|
Unit Size |
1 mg
|
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