APC-labeled anti-FLAG antibody, for capturing FLAG-tagged proteins and peptides in LANCE TR-FRET assays.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Mouse monoclonal antibody clone M2 against FLAG sequence conjugated to allophycocyanin (APC). APC is a fluorescent light harvesting protein unique to cyanobacteria and red algae and a member of the phycobiliprotein family of direct fluorescent dyes. Surelight APC is a chemically and intramolecularly cross-linked form of a protein. It is produced using a proprietary manufacturing process to increase thermal stability while maintaining the fluorescent and spectral properties of native dye. FLAG is a registered trademark of Sigma Aldrich Co.
LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).
Antibody Conjugates |
Anti-FLAG
|
---|---|
Application |
Protein-protein interactions
|
Automation Compatible |
Yes
|
Brand |
LANCE SureLight
|
Detection Method |
Time-Resolved Fluorescence (TRF)
TR-FRET
|
Experimental Type |
In vitro
|
Fluorophore |
SureLight APC
|
Format |
Microplates
|
Shipping Conditions |
Shipped in Blue Ice
|
Unit Size |
1 mg
|
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