The Alpha SureFire Ultra Multiplex p-Akt 1/2/3 (Ser473) (Eu) + Total Akt1 (Tb) assay kit is used to measure both phosphorylated and total levels of endogenous Akt in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated Akt1/2/3 analysis, and the 545 nm (Tb) signal corresponds to the total Akt1 analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

• No-wash steps, no separation steps
• Multiplexing assay
• Sensitive detection
• No interference from exogenous antibodies
• Results in less than 3 hours

In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.