Menu
US

PhenoVue Nile Red Lipid Stain

PhenoVue Nile Red Lipid Stain
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Nile Red
PhenoVue Nile Red Lipid Stain
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Nile Red
PhenoVue Nile Red Lipid Stain
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Nile Red
PhenoVue Nile Red Lipid Stain
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Nile Red

PhenoVue Nile Red Lipid stain is a lipophilic organic molecule commonly used for localization and quantification of intracellular lipid droplets. ​

PhenoVue Nile Red Lipid stain exhibits bright yellow fluorescence and is validated for use in imaging microscopy and high-content screening applications.

PhenoVue Nile Red Lipid stain exhibits:

  • maximum excitation wavelength of 512nm and maximum emission wavelength of 585 when complexed with triglycerides
  • maximum excitation wavelength of 552nm and maximum emission wavelength of 638nm when complexed with phospholipids

View our extensive validation data in the Product Information Sheet within the Resources tab below.

For research use only, not for use in diagnostic procedures.

Product information SDS, COAs, Manuals and more
Part number: CP41
List price: USD 174.38
Your price:
USD 174.38
USD 174.38 /each
Request more information

Product information

Overview

PhenoVue Nile Red Lipid stain is a lipophilic organic molecule that is almost nonfluorescent in water and polar solvents. In lipid-rich environments, Nile Red exhibits enhanced yellow fluorescence, as well as red fluorescence to a lesser extent.

Nile Red is commonly used for localization and quantification of intracellular lipid droplets which are ​involved in lipid synthesis, metabolism and transportation as well as protein storage and degradation or viral replication, all of which are related to pathophysiology, including dyslipidemia, obesity, lipodystrophy, diabetes, fatty liver diseases, or atherosclerosis.

Additional product information

Features
Numbers of vials per unit 2
Quantity or volume per vial 10mg (31.4µmoles)
Form Powder
Storage -20°C
Recommended working concentration 200nM (0.008ng/mL)​
Maximum excitation wavelength 512 nm - complexed with triglycerides
552 nm - complexed with phospholipids*
(*as shown in SpectraViewer below)
Maximum emission wavelength 585 nm - complexed with triglycerides
638 nm - complexed with phospholipids*
(*as shown in SpectraViewer below)
Common filter set Cy3.5
Live cell staining Yes
Fixed cell staining Yes. See Product Information Sheet for more information
Equivalent number of microplates 10900-32700​ x 96-well microplates
9090-32700​ x 384-well microplates
17040-40900 x 1536-well microplates

 

Specifications

Color
Yellow/Orange
Brand
PhenoVue™
Detection Method
Fluorescence
Filter
Cy3
Organelle and Cell Compartment
Lipid droplets
Product Compatibility
Live and fixed samples
Shipping Conditions
Shipped Ambient
Type
Individual Reagent
Unit Size
2 vials

Image gallery

PhenoVue Nile Red Lipid Stain
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Nile Red
PhenoVue Nile Red Lipid Stain
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Nile Red
PhenoVue Nile Red Lipid Stain
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Nile Red
PhenoVue Nile Red Lipid Stain
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Nile Red

Spectra Viewer

Resources

1-2 of 2 Resources
Flyer Icon
Flyer
PhenoVue Cellular Imaging Reagents Flyer

This flyer describes Revvity's PhenoVue cellular imaging reagents.

Learn More
Flyer Icon
Flyer
PhenoVue Reagents & Imaging Microplates Product List
Learn More
Scroll Icon