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PhenoVue Multi Organelle Staining Kit 1x384

PhenoVue Multi Organelle Staining Kit 1x384
HepG2 cells stained with PhenoVue 493 Lipid Stain after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 555- Goat Anti-Mouse Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system. View All
PhenoVue Multi Organelle Staining Kit 1x384
PhenoVue Multi Organelle Staining Kit 1x384
HepG2 cells stained with PhenoVue 493 Lipid Stain after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 555- Goat Anti-Mouse Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Multi Organelle Staining Kit after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 400LS Phalloidin after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 647 - Goat Anti-Rat Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
PhenoVue Multi Organelle Staining Kit 1x384
HepG2 cells stained with PhenoVue 493 Lipid Stain after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 555- Goat Anti-Mouse Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Multi Organelle Staining Kit after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 400LS Phalloidin after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 647 - Goat Anti-Rat Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
PhenoVue Multi Organelle Staining Kit 1x384
PhenoVue Multi Organelle Staining Kit 1x384
HepG2 cells stained with PhenoVue 493 Lipid Stain after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 555- Goat Anti-Mouse Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Multi Organelle Staining Kit after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 400LS Phalloidin after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 647 - Goat Anti-Rat Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
PhenoVue Multi Organelle Staining Kit 1x384
HepG2 cells stained with PhenoVue 493 Lipid Stain after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 555- Goat Anti-Mouse Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Multi Organelle Staining Kit after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 400LS Phalloidin after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 647 - Goat Anti-Rat Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.

PhenoVue™ Multi Organelle Staining Kit comprises five validated, optimized and ready-to-use fluorescent probes to visualize mitochondria, lysosomes, lipid droplets, actin cytoskeleton, and nuclei in a 5-plex experiment.

The kit is validated for use on high-content screening systems such as the Opera Phenix® Plus high content screening system, and is ideal for the study of metabolic disorders, infectious diseases, and liver toxicity.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

For research use only. Not for use in diagnostic procedures.

View product information
Request More Information

PhenoVue™ Multi Organelle Staining Kit comprises five validated, optimized and ready-to-use fluorescent probes to visualize mitochondria, lysosomes, lipid droplets, actin cytoskeleton, and nuclei in a 5-plex experiment.

The kit is validated for use on high-content screening systems such as the Opera Phenix® Plus high content screening system, and is ideal for the study of metabolic disorders, infectious diseases, and liver toxicity.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

For research use only. Not for use in diagnostic procedures.

View product information
Product Variant
Quantity: 1 kit
Part #:
PMOS11
List Price
USD 612.42
PhenoVue Multi Organelle Staining Kit 1x384
HepG2 cells stained with PhenoVue 493 Lipid Stain after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 555- Goat Anti-Mouse Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Multi Organelle Staining Kit after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 400LS Phalloidin after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 647 - Goat Anti-Rat Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
PhenoVue Multi Organelle Staining Kit 1x384
HepG2 cells stained with PhenoVue 493 Lipid Stain after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 555- Goat Anti-Mouse Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Multi Organelle Staining Kit after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 400LS Phalloidin after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 647 - Goat Anti-Rat Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
PhenoVue Multi Organelle Staining Kit 1x384
PhenoVue Multi Organelle Staining Kit 1x384
HepG2 cells stained with PhenoVue 493 Lipid Stain after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 555- Goat Anti-Mouse Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Multi Organelle Staining Kit after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 400LS Phalloidin after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 647 - Goat Anti-Rat Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
PhenoVue Multi Organelle Staining Kit 1x384
HepG2 cells stained with PhenoVue 493 Lipid Stain after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 555- Goat Anti-Mouse Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Multi Organelle Staining Kit after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 400LS Phalloidin after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 647 - Goat Anti-Rat Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
PhenoVue Multi Organelle Staining Kit 1x384
PhenoVue Multi Organelle Staining Kit 1x384
PhenoVue Multi Organelle Staining Kit 1x384
HepG2 cells stained with PhenoVue 493 Lipid Stain after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 555- Goat Anti-Mouse Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Multi Organelle Staining Kit after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 400LS Phalloidin after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 647 - Goat Anti-Rat Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
PhenoVue Multi Organelle Staining Kit 1x384
HepG2 cells stained with PhenoVue 493 Lipid Stain after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 555- Goat Anti-Mouse Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Multi Organelle Staining Kit after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 400LS Phalloidin after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 647 - Goat Anti-Rat Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.

Product information

Overview

PhenoVue Multi Organelle Staining Kit includes five PhenoVue probes to visualize nuclei, mitochondria, lysosomes, actin, and lipid droplets. These organelles play crucial roles in various cellular processes and interact with each other in intricate ways to maintain cell homeostasis and carry out specific cellular processes.

The combination of specific, bright and photostable PhenoVue dyes enables the ability to perform a 5-plex experiment with maximum spectral separation and a significantly reduced spectral overlap.

Each PhenoVue probe has been extensively validated and carefully optimized for excellent batch-to-batch reproducibility.

The PhenoVue Multi Organelle Staining Kit offers a straightforward and user-friendly protocol to facilitate the experimental procedure. Avoiding the use of living cells, it provides a convenient alternative method for phenotypic screening approaches such as the well-established cell painting protocol.

Kit Components / Quantity:

  • PhenoVue Hoechst 33342 Nuclear Stain / 1 vial of 70 µL (50000x)
  • PhenoVue 493 Lipid Stain / 1 vial of 30 µL (4000x)
  • PhenoVue Fluor 400LS - Phalloidin / 1 vial of 30 µL (400X)
  • PhenoVue anti-HSP60 antibody 100X / 1 vial of 100 µL (100x)
  • PhenoVue anti-LAMP1 antibody 100X / 1 vial of 100 µL (100x)
  • PhenoVue Fluor 555 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed / 1 vial of 100 µL (100x)
  • PhenoVue Fluor 647 - Goat Anti-Rat Antibody Highly Cross-Adsorbed / 1 vial of 50 µL (200x)
  • PhenoVue Dye Diluent A (5X) / 1 vial - 8 mL (5X)

Specifications

Application
High Content Imaging
Microscopy
Assay Points
1 x 384-well microplate
Brand
PhenoVue™
Detection Modality
Fluorescence
Organelle and Cell Compartment
Actin
Lipid droplets
Lysosomes
Mitochondria
Nuclei
Quantity
1 kit
Sample Type
Fixed samples only
Shipping Conditions
Shipped in Dry Ice
Storage Conditions
-16 °C or below, protected from light
Type
Kit

Image gallery

PhenoVue Multi Organelle Staining Kit 1x384
HepG2 cells stained with PhenoVue 493 Lipid Stain after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 555- Goat Anti-Mouse Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Multi Organelle Staining Kit after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 400LS Phalloidin after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 647 - Goat Anti-Rat Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
PhenoVue Multi Organelle Staining Kit 1x384
HepG2 cells stained with PhenoVue 493 Lipid Stain after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 555- Goat Anti-Mouse Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Multi Organelle Staining Kit after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 400LS Phalloidin after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 647 - Goat Anti-Rat Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
PhenoVue Multi Organelle Staining Kit 1x384
PhenoVue Multi Organelle Staining Kit 1x384
HepG2 cells stained with PhenoVue 493 Lipid Stain after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 555- Goat Anti-Mouse Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Multi Organelle Staining Kit after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 400LS Phalloidin after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 647 - Goat Anti-Rat Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
PhenoVue Multi Organelle Staining Kit 1x384
HepG2 cells stained with PhenoVue 493 Lipid Stain after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 555- Goat Anti-Mouse Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Multi Organelle Staining Kit after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 400LS Phalloidin after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 647 - Goat Anti-Rat Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
PhenoVue Multi Organelle Staining Kit 1x384
PhenoVue Multi Organelle Staining Kit 1x384
HepG2 cells stained with PhenoVue 493 Lipid Stain after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 555- Goat Anti-Mouse Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Multi Organelle Staining Kit after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 400LS Phalloidin after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 647 - Goat Anti-Rat Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
PhenoVue Multi Organelle Staining Kit 1x384
HepG2 cells stained with PhenoVue 493 Lipid Stain after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 555- Goat Anti-Mouse Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Multi Organelle Staining Kit after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 400LS Phalloidin after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.
HepG2 cells stained with PhenoVue Fluor 647 - Goat Anti-Rat Antibody after oleic acid treatment, imaged on the Opera Phenix Plus HCS system.

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