PhenoVue Fluor 647 - Concanavalin A is a fluorescent lectin which displays high affinity for glycoproteins and glycolipids present at the cellular membranes. It can be used for cellular membrane staining, particularly the endoplasmic reticulum. PhenoVue Fluor 647 - Concanavalin A exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications. Part of Revvity's portfolio of cellular imaging reagents, PhenoVue Fluor 647 - Concanavalin A has a maximum excitation wavelength of 650 nm and a maximum emission wavelength of 670 nm.
View our extensive validation data in the Product Information Sheet within the Resources tab below.
For research use only, not for use in diagnostic procedures.
Concanavalin A is a plant homotetrameric lectin known to activate the immune system or induce apoptosis and autophagy. Concanavalin A displays high affinity for α-mannopyranosyl and α-glucopyranosyl residues of glycoproteins and glycolipids present at the cellular membranes. Fluorescent Concanavalin A derivatives are commonly used for staining the cellular membranes of mammalian cells, particularly the endoplasmic reticulum. PhenoVue Fluor 647 - Concanavalin A can be used to visualize cellular membranes in immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
Numbers of vials per unit | 5 |
---|---|
Quantity or volume per unit | 1mg (9.62nmoles) |
Form | Lyophilized |
Storage | 2 - 8°C |
Recommended working concentration | 100 µg/mL |
Maximum excitation wavelength | 650 nm |
Maximum emission wavelength | 670 nm |
Common filter set | Cy5 |
Live cell staining | Yes |
Fixed cell staining | Yes. See Product Information Sheet for more information |
Equivalent number of microplates | 1 - 5 x 96-well microplates 1 - 5 x 384-well microplates 3 - 8 x 1536-well microplates |
Color |
Red
|
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Brand |
PhenoVue™
|
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Detection Method |
Fluorescence
|
Filter |
Cy5
|
Fluorophore |
PhenoVue™ Fluor 647
|
Organelle and Cell Compartment |
Endoplasmic Reticulum
Plasma membranes
|
Product Compatibility |
Live and fixed samples
|
Shipping Conditions |
Shipped Ambient
|
Type |
Individual Reagent
|
Unit Size |
5 vials
|
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