PhenoVue Fluor 594 - Phalloidin

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PhenoVue Fluor 594 - Phalloidin

PhenoVue Fluor 594 - Phalloidin is a fluorescent dye conjugated bicyclic peptide which binds specifically to F-actin and stains the actin cytoskeleton.

PhenoVue Fluor 594 conjugated Phalloidin exhibits bright red fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of Revvity's portfolio of cellular imaging reagents, PhenoVue 594 - Phalloidin has a maximum excitation wavelength of 590 nm and a maximum emission wavelength of 617 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

For research use only, not for use in diagnostic procedures.

Product information

SDS, COAs, Manuals and more

Product information

Overview

Phalloidin is a toxin isolated from the fungus Amanita phalloides. It is a bicyclic peptide that specifically binds and stabilizes actin filaments.

Fluorescent Phalloidin derivatives have been extensively used for monitoring actin expression and dynamics of the cytoskeleton network. The study of actin cytoskeleton regulation is of particular interest in the context of several pathophysiological processes such as cancer and neurodegenerative diseases as well as fibrosis and vascular diseases.

PhenoVue Fluor 594 - Phalloidin can be used to vizualise actin filaments in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.

Additional product information

Features

Numbers of vials per unit	1
Quantity or volume per vial	300 units (10nmoles; 13µg)
Form	Dessicated
Storage	-20°C
Recommended working concentration	165nM (0.21µg/mL)
Maximum excitation wavelength	590 nm
Maximum emission wavelength	617 nm
Common filter set	FITC
Live cell staining	No
Fixed cell staining	Yes
Equivalent number of microplates	2-6 x 96-well microplates 1.5-6 x 384-well microplates 3-10 x 1536-well microplates

Specifications

Physical Specifications

Color	Red

Other Specifications

Brand	PhenoVue^™
Detection Method	Fluorescence
Filter	Texas-Red
Fluorophore	PhenoVue^™ Fluor 594
Organelle and Cell Compartment	Actin
Product Compatibility	Fixed samples only
Shipping Conditions	Shipped in Dry Ice
Type	Individual Reagent
Unit Size	1 vial