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PhenoVue Fluor 555 - Phalloidin
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PhenoVue Fluor 555 - Phalloidin
PhenoVue™ Fluor 555 - Phalloidin is a fluorescent dye conjugated bicyclic peptide which binds specifically to F-actin and stains the actin cytoskeleton.
PhenoVue Fluor 555 conjugated Phalloidin exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications.
Part of Revvity's portfolio of cellular imaging reagents, PhenoVue 555 - Phalloidin has a maximum excitation wavelength of 555 nm and a maximum emission wavelength of 570 nm.
View our extensive validation data in the Product Information Sheet within the Resources tab below.
For research use only. Not for use in diagnostic procedures.
| Feature | Specification |
|---|---|
| Color | Yellow |
| Filter | Cy3 |
| Fluorophore | PhenoVue™ Fluor 555 |
| Organelle and Cell Compartment | Actin |
PhenoVue™ Fluor 555 - Phalloidin is a fluorescent dye conjugated bicyclic peptide which binds specifically to F-actin and stains the actin cytoskeleton.
PhenoVue Fluor 555 conjugated Phalloidin exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications.
Part of Revvity's portfolio of cellular imaging reagents, PhenoVue 555 - Phalloidin has a maximum excitation wavelength of 555 nm and a maximum emission wavelength of 570 nm.
View our extensive validation data in the Product Information Sheet within the Resources tab below.
For research use only. Not for use in diagnostic procedures.
PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
Product information
Overview
Phalloidin is a toxin isolated from the fungus Amanita phalloides. It is a bicyclic peptide that specifically binds and stabilizes actin filaments.
Fluorescent Phalloidin derivatives have been extensively used for monitoring actin expression and dynamics of the cytoskeleton network. The study of actin cytoskeleton regulation is of interest in the context of several pathophysiological processes such as cancer and neurodegenerative diseases as well as fibrosis and vascular diseases.
PhenoVue Fluor 555 - Phalloidin can be used to vizualise actin filaments in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
Additional product information
Features
| Numbers of vials per unit | 1 |
|---|---|
| Quantity or volume per unit | 300 units (10nmoles; 13µg) |
| Form | Dessicated |
| Storage | -20°C |
| Recommended working concentration | 165nM (0.21µg/mL) |
| Maximum excitation wavelength | 555nm |
| Maximum emission wavelength | 570nm |
| Common filter set | Cy3 |
| Live cell staining | No |
| Fixed cell staining | Yes |
| Equivalent number of microplates | 2 - 6 x 96-well microplates 1.5 - 6 x 384-well microplates 3 - 10 x 1536-well microplates |
Specifications
| Color |
Yellow
|
|---|---|
| Form |
Desiccated
|
| Maximum Emission Wavelength (Emmax) |
570 nm
|
| Maximum Excitation Wavelength (Exmax) |
555 nm
|
| Application |
High Content Imaging
Microscopy
|
|---|---|
| Brand |
PhenoVue™
|
| Detection Modality |
Fluorescence
|
| Filter |
Cy3
|
| Fluorophore |
PhenoVue™ Fluor 555
|
| Organelle and Cell Compartment |
Actin
|
| Quantity |
1 x 300 units
|
| Sample Type |
Fixed samples only
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Storage Conditions |
-16 °C or below, protected from light
|
| Type |
Individual Reagent
|
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PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
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Resources
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