PhenoVue Fluor 488 - Concanavalin A is a fluorescent lectin which displays high affinity for glycoproteins and glycolipids present at the cellular membranes. It can be used for cellular membrane staining, particularly the endoplasmic reticulum.
PhenoVue Fluor 488 - Concanavalin A exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications.
Part of Revvity's portfolio of cellular imaging reagents, PhenoVue Fluor 488 - Concanavalin A has a maximum excitation wavelength of 495 nm and a maximum emission wavelength of 520 nm.
View our extensive validation data in the Product Information Sheet within the Resources tab below.
For research use only, not for use in diagnostic procedures.
Concanavalin A is a plant homotetrameric lectin known to activate the immune system or induce apoptosis and autophagy. Concanavalin A displays high affinity for α-mannopyranosyl and α-glucopyranosyl residues of glycoproteins and glycolipids present at the cellular membranes.
Fluorescent Concanavalin A derivatives are commonly used for staining the cellular membranes of mammalian cells, particularly the endoplasmic reticulum.
PhenoVue Fluor 488 - Concanavalin A can be used to visualize cellular membranes in immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
Numbers of vials per unit | 5 |
---|---|
Quantity or volume per vial | 1mg (29.2nmoles) |
Form | Lyophilized |
Storage | 2-8°C |
Recommended working concentration | 1mg (9.62nmoles) |
Maximum excitation wavelength | 495 nm |
Maximum emission wavelength | 520 nm |
Common filter set | FITC |
Live cell staining | Yes |
Fixed cell staining | Yes. See Product Information Sheet for more information |
Equivalent number of microplates | 1-5 x 96-well microplates 1-5 x 384-well microplates 3-8 x 1536-well microplates |
Color |
Green
|
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Brand |
PhenoVue™
|
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Detection Method |
Fluorescence
|
Filter |
FITC
|
Fluorophore |
PhenoVue™ Fluor 488
|
Organelle and Cell Compartment |
Endoplasmic Reticulum
Plasma membranes
|
Product Compatibility |
Live and fixed samples
|
Shipping Conditions |
Shipped Ambient
|
Type |
Individual Reagent
|
Unit Size |
5 vials
|
Fluorescent Concanavalin A conjugates represent a method of choice for labelling the cellular membranes of mammalian cells...
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