PhenoVue DRAQ5 Total Cell Nuclear Stain is a lipophilic and membrane permeable far-red fluorescent DNA stain for live or fixed cells. Unlike the routinely used Hoechst 33342, PhenoVue DRAQ5 does not require UV excitation and has little spectral overlap with visible range chromophores. This property makes it readily used for multiplexing applications with other fluorophores even with rhodamine-derived probes. It is spectrally compatible with simple viability dyes such as propidium iodide, and more sophisticated functional probes such as PhenoVue ROS-490 Reactive Oxygen species indicator, PhenoVue 551 Mitochondrial Stain, or PhenoVue Fluor 488 and 555 conjugates, as well as CFP, GFP and YFP tagged proteins. This total cell nuclear stain allows robust cell event counting, while offering nuclear morphology, insights on DNA condensation, fragmentation, and cytoplasmic morphology from a weak differential staining of this compartment. PhenoVue DRAQ5 is preferentially excited by red wavelengths, with a maximum excitation wavelength at 646 nm. Its maximum emission peaks at 697 nm when bound to double stranded DNA. This stain is provided in an aqueous, ready-to-use solution which is usually added as the final step in a staining protocol. It is documented in HCS applications on imaging platforms.

Features:

• Numbers of Vials Per Unit: 1
• Quantity or Volume Per Vial: 50 µL (5 mM – 250 nmoles)
• Form: Liquid
• Storage: 2-8 °C (Do not freeze)
• Recommended Working Concentration: 5 µM
• Maximum Excitation Wavelength: 646 nm
• Maximum Emission Wavelength: 697 nm
• Common Filter Set: Cy5
• Live Cell Staining: Yes
• Fixed Cell Staining: Yes

Equivalent Number of Microplates:

• 2-5 x 96-well microplates
• 2-5 x 384-well microplates
• 3-8 x 1536-well microplates