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NEXTFLEX™ Unique dual index barcodes (10NT, 1-48)

Multiplex up to 1,536 samples on a single flow cell with NEXTFLEX™ Unique Dual Index Barcodes (10NT UDIs). Each kit ships with up to 1,536 pre-validated i5/i7 index pairs that eliminate index hopping and are compatible with Illumina platforms such as the NovaSeq® and Element’s AVITI™ systems. Off-the-shelf plate sets (containing between 16 to 1,536 unique adapters) let you start small and scale to population-level projects without requiring custom ordering or re-balancing.

Feature Specification
Automation Compatible Yes
Product Group Barcodes

Multiplex up to 1,536 samples on a single flow cell with NEXTFLEX™ Unique Dual Index Barcodes (10NT UDIs). Each kit ships with up to 1,536 pre-validated i5/i7 index pairs that eliminate index hopping and are compatible with Illumina platforms such as the NovaSeq® and Element’s AVITI™ systems. Off-the-shelf plate sets (containing between 16 to 1,536 unique adapters) let you start small and scale to population-level projects without requiring custom ordering or re-balancing.

Product variants
Barcodes: 1 - 16
Format
1 rxn per well
Unit Size
16 rxns
Part #:
NOVA-634100-EVAL16
List price
USD 108.12
Your online price:
Barcodes: 1 - 24
Format
1 rxn per well
Unit Size
24 rxns
Part #:
NOVA-634100-EVAL24
List price
USD 158.00
Your online price:
Barcodes: 1 - 48
Format
1 rxn per well
Unit Size
48 rxns
Part #:
NOVA-634100-EVAL48
List price
USD 323.34
Your online price:
Barcodes: 1 - 96
Format
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634101
List price
USD 646.68
Your online price:
Barcodes: 97 - 192
Format
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634102
List price
USD 646.68
Your online price:
Barcodes: 193 - 288
Format
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634103
List price
USD 646.68
Your online price:
Barcodes: 289 - 384
Format
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634104
List price
USD 646.68
Your online price:
Barcodes: 385 - 480
Format
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634105
List price
USD 646.68
Your online price:
Barcodes: 481 - 576
Format
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634106
List price
USD 646.68
Your online price:
Barcodes: 577 - 672
Format
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634107
List price
USD 646.68
Your online price:
Barcodes: 673 - 768
Format
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634108
List price
USD 646.68
Your online price:
Barcodes: 769 - 864
Format
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634109
List price
USD 646.68
Your online price:
Barcodes: 865 - 960
Format
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634110
List price
USD 646.68
Your online price:
Barcodes: 961 - 1056
Format
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634111
List price
USD 646.68
Your online price:
Barcodes: 1057 - 1152
Format
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634112
List price
USD 646.68
Your online price:
Barcodes: 1153 - 1248
Format
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634113
List price
USD 646.68
Your online price:
Barcodes: 1249 - 1344
Format
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634114
List price
USD 646.68
Your online price:
Barcodes: 1345 - 1440
Format
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634115
List price
USD 646.68
Your online price:
Barcodes: 1441 - 1536
Format
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634116
List price
USD 646.68
Your online price:
Barcodes: 1 - 1536
Format
1 rxn per well
Unit Size
1,536 rxns
Part #:
NOVA-634100
List price
USD 10,327.50
Your online price:
Barcodes: 1 - 384
Format
2 rxns per well
Unit Size
768 rxns
Part #:
NOVA-534101
List price
USD 5,164.26
Your online price:
Barcodes: 385 - 768
Format
2 rxns per well
Unit Size
768 rxns
Part #:
NOVA-534102
List price
USD 5,164.26
Your online price:
Barcodes: 769 - 1152
Format
2 rxns per well
Unit Size
768 rxns
Part #:
NOVA-534103
List price
USD 5,164.26
Your online price:
Barcodes: 1153 - 1536
Format
2 rxns per well
Unit Size
768 rxns
Part #:
NOVA-534104
List price
USD 5,164.26
Your online price:
Barcodes: 1 - 1,536
Format
2 rxns per well
Unit Size
3,072 rxns
Part #:
NOVA-534100
List price
USD 20,655.00
Your online price:
For research use only. Not for use in diagnostic procedures.

Overview

  • Up to 1,536 unique i5 / i7 pairs (10NT) ready to ship
  • Compatible with PCR-free and PCR-amplified workflows
  • ≥ 3-base Hamming distance across the entire set for robust error-correction and balanced base diversity on 2- & 4-color chemistries
  • Designed to minimize index hopping that can be present with patterned flow cells.
  • Compatible with Revvity library prep and TruSeq style, ligation-based workflows
  • Every lot is functionally validated and tested for index purity by sequencing in an ISO 9001 facility
  • Optional NEXTFLEX Universal Blockers boost on-target hybrid-capture efficiency when used with these UDIs.

Additional product information

NGS Multiplexing Performance

Scale from 2 to 1,536 libraries in a single NovaSeq® (Illumina) or AVITI® (Element)flow cell. The 10NT UDI set supports barcode rotation between runs, eliminating carry-over and virtually stopping index hopping on patterned flow cells. Adapters work out-of-the-box with all TruSeq-style library-prep kits, including NEXTFLEX, Illumina, Watchmaker, Twist library prep kit s, whether PCR-free or PCR-amplified.

“Revvity has a set of 1536 validated UDI adapters that we have been regularly using for 5 years. Using the Illumina® NovaSeq® X Plus with this UDI set from Revvity we can sequencing 12,288 samples across 8 lanes of the 10B or 25B flow cells.  This dramatically lowers our sequencing cost per sample!”

-Dr. Charlie Johnson, Director of Texas A&M Agri Life Genomics and Bioinformatics Service

NGS Adapters with High Data Integrity

Each NEXTFLEX UDI adapter has a dedicated 10NT i7 & i5 sequence separated from every other pair by a ≥ 3-base Hamming distance, enabling single-base error correction during demultiplexing. Because the adapter already contains the full flow-cell and primer-binding sites, PCR enrichment is optional. On patterned flow cells (Illumina NovaSeq®, HiSeq® 3000/4000, HiSeq® X, Element AVITI™) the use of true, unique dual indexes cuts index-hopping artefacts to background levels by removing mis-assigned reads. Every lot is sequenced for ≥ 99.9 % index purity and functional ligation efficiency, ensuring data integrity run after run.

nextflex udi barcodes infographic wide
 

Specifications

Automation Compatible
Yes
Barcodes
1 - 48
Format
1 rxn per well
Product Group
Barcodes
Shipping Conditions
Shipped in Dry Ice
Unit Size
48 rxns

References

Selected Citations that Reference the Use of the NEXTFLEX UDI Barcodes:

  • Adolfi, A., Gantz, V.M., Jasinskiene, N. et al. Efficient population modification gene-drive rescue system in the malaria mosquito Anopheles stephensi. Nat Commun 11, 5553 (2020).
  • Estermann, M. (2020). Mouse embryonic stem cells self-organize into trunk-like structures with neural tube and somites. doi:10.1242/prelights.18906.
  • Gardner, E. J., Prigmore, E., Gallone, G., Danecek, P., Samocha, K. E., Handsaker, J., . . . Hurles, M. E. (2019). Contribution of retrotransposition to developmental disorders. Nature Communications, 10(1). doi:10.1038/s41467-019-12520-y.
  • Gaeta, N. C., Bean, E., Miles, A. M., Daniel Ubriaco Oliveira Gonçalves De Carvalho, Alemán, M. A., Carvalho, J. S., . . . Ganda, E. (2020). A Cross-Sectional Study of Dairy Cattle Metagenomes Reveals Increased Antimicrobial Resistance in Animals Farmed in a Heavy Metal Contaminated Environment. Frontiers in Microbiology, 11. doi:10.3389/fmicb.2020.590325.
  • Hirose, K., Chang, S., Yu, H., Wang, J., Barca, E., Chen, X., . . . Huang, G. N. (2019). Loss of a novel striated muscle-enriched mitochondrial protein Coq10a enhances postnatal cardiac hypertrophic growth. doi:10.1101/755793.
  • Leon, K. E., et al. (2020) DOT1L modulates the senescence-associated secretory phenotype through epigenetic regulation of IL1A. bioRxiv 2020.08.21.258020; doi: 10.1101/2020.08.21.258020
  • Miura, H., Takahashi, S., Shibata, T. et al. Mapping replication timing domains genome wide in single mammalian cells with single-cell DNA replication sequencing. Nat Protoc 15, 4058–4100 (2020). doi.org/10.1038/s41596-020-0378-5
  • Starr, T. N., Greaney, A. J., Hilton, S. K., Crawford, K. H., Navarro, M. J., Bowen, J. E., Bloom, J. D. (2020). Deep mutational scanning of SARS-CoV-2 receptor binding domain reveals constraints on folding and ACE2 binding. doi:10.1101/2020.06.17.157982.
  • Veenvliet, J. V., et al. (2020) Mouse embryonic stem cells self-organize into trunk-like structures with neural tube and somites. bioRxiv 2020.03.04.974949; doi.org/10.1101/2020.03.04.974949.
  • Dubey SK et al. (2025). Deciphering age-related transcriptomic changes in the mouse retinal pigment epithelium. Aging (Albany NY) 17(3):657–684. DOI: 10.18632/aging.206219
  • Pujari & Cullen (2024) Modulators of MAPK pathway activity during filamentous growth in Saccharomyces cerevisiae. G3 14(6):jkae072
  • Kant et al. (2023) High-quality full genome assembly of historic Xylella fastidiosa strains… Microbiol Resour Announc. 12(11):e00536-23.
  • Arras et al. (2023) Characterisation of an E. coli line that completely lacks ribonucleotide reduction… eLife 12:e83845.
  • Larkin et al. (2025) Climate-driven succession in marine microbiome biodiversity and biogeochemical function. Nat Commun. 16:3926.
  • Klauer et al. (2024) Hydrophobins from Aspergillus mediate fungal interactions with microplastics. bioRxiv preprint 2024.11.05.622132.

FAQs

  • Are the UDI barcodes sequence-verified and functionally validated?
  • What is the lead time when I order the NEXTFLEX unique dual index barcodes?
  • Must I perform paired-end sequencing with NEXTFLEX Unique Dual Index Barcodes?
  • Can I use the NEXTFLEX Unique Dual Index Barcodes in single index experiments?
  • What are the storage and shipping conditions?
  • Do the UDIs work with PCR-free workflows?
  • Can I combine just a subset of the indexes in one run?
  • How do unique dual indexes mitigate index hopping?
  • Are these adapters compatible with NEXTFLEX Universal Blockers? 

Resources

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NEXTFLEX UDI Barcodes Sequences 1536 Set

This excel file includes the sequences of the indexes for the 1536 NEXTFLEX UDI Barcodes.

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