
Feature | Specification |
---|---|
Automation Compatible | Yes |
Product Group | Barcodes |
Scale from 2 to 1,536 libraries in a single NovaSeq® (Illumina) or AVITI® (Element)flow cell. The 10NT UDI set supports barcode rotation between runs, eliminating carry-over and virtually stopping index hopping on patterned flow cells. Adapters work out-of-the-box with all TruSeq-style library-prep kits, including NEXTFLEX, Illumina, Watchmaker, Twist library prep kit s, whether PCR-free or PCR-amplified.
“Revvity has a set of 1536 validated UDI adapters that we have been regularly using for 5 years. Using the Illumina® NovaSeq® X Plus with this UDI set from Revvity we can sequencing 12,288 samples across 8 lanes of the 10B or 25B flow cells. This dramatically lowers our sequencing cost per sample!”
-Dr. Charlie Johnson, Director of Texas A&M Agri Life Genomics and Bioinformatics Service
Each NEXTFLEX UDI adapter has a dedicated 10NT i7 & i5 sequence separated from every other pair by a ≥ 3-base Hamming distance, enabling single-base error correction during demultiplexing. Because the adapter already contains the full flow-cell and primer-binding sites, PCR enrichment is optional. On patterned flow cells (Illumina NovaSeq®, HiSeq® 3000/4000, HiSeq® X, Element AVITI™) the use of true, unique dual indexes cuts index-hopping artefacts to background levels by removing mis-assigned reads. Every lot is sequenced for ≥ 99.9 % index purity and functional ligation efficiency, ensuring data integrity run after run.
Automation Compatible |
Yes
|
---|---|
Barcodes |
1 - 48
|
Format |
1 rxn per well
|
Product Group |
Barcodes
|
Shipping Conditions |
Shipped in Dry Ice
|
Unit Size |
48 rxns
|
Selected Citations that Reference the Use of the NEXTFLEX UDI Barcodes:
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This excel file includes the sequences of the indexes for the 1536 NEXTFLEX UDI Barcodes.
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