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Multiplex Surefire Ultra Phospho (Tyr701)/Total STAT1 Assay Kit, 100 Assay Points
The AlphaLISA® SureFire® Ultra™ Multiplex phospho-STAT1 (Tyr701) + Total STAT1 assay kit is used to measure both the phosphorylation of STAT1 and total levels of endogenous STAT1 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure STAT1 phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product information
Overview
The Alpha SureFire®Ultra™ Multiplex p-STAT1 (Tyr701) (Eu) + Total STAT1 assay kit is used to measure both phosphorylated and total levels of endogenous STAT1 in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated STAT1 analysis, and the 545 nm (Tb) signal corresponds to the total STAT1 analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.
- No-wash steps, no separation steps
- Multiplexing assay
- Sensitive detection
- No interference from exogenous antibodies
- Results in less than 3 hours
In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.
Specifications
| Application |
Protein Detection
|
|---|---|
| Assay Points |
100
|
| Assay Target Class |
Phospho-protein
Protein
|
| Assay Technology |
Alpha
|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra Multiplex
|
| Detection Method |
Alpha
|
| Experimental Type |
In vitro
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Therapeutic Area |
Inflammation
Oncology
|
| Unit Size |
100 Assay Points
|
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Video gallery
Resources
This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...
This Quick Start Guide is intended to provide the Alpha user with the knowledge and tools necessary to quickly and easily unleash...
An in-depth review of molecular and cellular pathways
The maintenance of proteostasis, the biological mechanisms that control the...
SDS, COAs, Manuals and more
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