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HTRF Tag-Lite Vasopressin Receptor Red Antagonist Ligand, 5,000 Assay Points

This Vasopressin receptor red antagonist is a Vasopressin V2 Benzazepine derivative labeled with a red emitting HTRF fluorescent probe.

For research use only. Not for use in diagnostic procedures.

Feature Specification
Application Receptor-Ligand Binding

This Vasopressin receptor red antagonist is a Vasopressin V2 Benzazepine derivative labeled with a red emitting HTRF fluorescent probe.

For research use only. Not for use in diagnostic procedures.

Product Variant
Unit Size: 5,000 Assay Points
Part #:
L0063RED
List Price
USD 1,353.00

Overview

Tag-lite receptor binding assays use either a green or a red fluorescent ligand to function.

This Vasopressin receptor red antagonist is a Vasopressin V2 Benzazepine derivative labeled with a red emitting HTRF fluorescent probe.

This ligand is best suited for use in the Vasopressin/Oxytocin V2 receptor binding assay.

Specifications

Application
Receptor-Ligand Binding
Brand
Tag-lite
Detection Modality
HTRF
Product Group
Fluorescent Reagent
Shipping Conditions
Shipped in Dry Ice
Target Class
GPCR
Technology
TR-FRET
Therapeutic Area
Cardiovascular
Infectious Diseases
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
Rare Diseases
Unit Size
5,000 Assay Points

Video gallery

How it works

Assay principle

Running a receptor binding assay using Tag-lite is as easy as it can get. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right. Running a receptor binding assay using Tag-lite is as easy as it can get. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.

gpcr-how-it-works-assay-principle-receptor-binding-l0063red
Saturation binding (KD)

A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.

gpcr-how-it-works-saturation-binding-kd-l0063red
how-it-works-vasopressin-receptor-red-antagonist
Competitive binding (KI)

A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.

gpcr-how-it-works-competitive-binding-ki-l0063red
how-it-works-vasopressin-receptor-red-antagonist

 

Resources

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