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HTRF Testosterone Detection Kit, 500 Assay Points
HTRF Testosterone Detection Kit, 500 Assay Points
PHOTO TESTOSTERONE KIT
The Testosterone kit is designed for the fast quantification of the steroid hormone in cell-based and enzymatic formats.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
| Feature | Specification |
|---|---|
| Application | Protein Quantification |
| Sample Volume | 10 µL |
The Testosterone kit is designed for the fast quantification of the steroid hormone in cell-based and enzymatic formats.
Product Variants
Unit Size: 500 Assay Points
Part #:
64TESPEG
List Price
USD 852.84
Unit Size: 10,000 Assay Points
Part #:
64TESPEH
List Price
USD 9,232.00
HTRF Testosterone Detection Kit, 500 Assay Points
PHOTO TESTOSTERONE KIT
HTRF Testosterone Detection Kit, 500 Assay Points
Product information
Overview
Testosterone is a steroid hormone synthesized from 17a-hydroxy progesterone by the enzymes 17,20-lyase and 17ß-hydroxysteroid dehydrogenase in the gonads of both males and females, although small amounts are also secreted by the adrenal glands. It is the principal male sex hormone and an anabolic steroid. It plays an important role in the stimulation and the development of masculine characteristics by its fixation of androgen receptors.
Specifications
| Application |
Protein Quantification
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Kit
|
| Sample Volume |
10 µL
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
Biomarkers
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Metabolism/Diabetes
Neuroscience
|
| Unit Size |
500 Assay Points
|
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HTRF Testosterone Detection Kit, 500 Assay Points
HTRF Testosterone Detection Kit, 500 Assay Points
How it works
Assay principle
The testosterone assay is based on the competition principle, where native testosterone produced by cells and XL665-labelled testosterone compete for binding to a monoclonal anti-testosterone antibody labelled with Europium Cryptate.
Assay Protocol
The testosterone assay features a streamlined protocol with only two incubation steps: - Cell stimulation by target compounds - Testosterone detection using HTRF reagents This protocol requires a single one-hour incubation period following cell stimulation.
Assay details
Key features
| Detection limit: | 0.1 nM |
| Dynamic range: | 0.1 to 10 nM |
| EC50 | 0.84 nM |
| S/B | 13 |
| Specificity: | low cross-reactivity with 4-Androstene-3,17-Dione 1.3% |
Resources
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Brochure
HTRF assays and reagents catalog
Discover the versatility and precision of Homogeneous Time-Resolved Fluorescence (HTRF) technology. Our HTRF portfolio offers a...
Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
SDS, COAs, Manuals and more
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Safety data sheet
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