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HTRF Tau Aggregation Kit, 10,000 Assay Points
HTRF Tau Aggregation Kit, 10,000 Assay Points
HTRF Tau Aggregation aggregation Detection Kit
The Tau Aggregation kit is designed for the fast detection of human Tau aggregates in brain tissues and cell cultures.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
| Feature | Specification |
|---|---|
| Application | Protein Quantification |
| Sample Volume | 10 µL |
The Tau Aggregation kit is designed for the fast detection of human Tau aggregates in brain tissues and cell cultures.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Unit Size: 500 Assay Points
Part #:
6FTAUPEG
List Price
USD 1,210.00
Unit Size: 10,000 Assay Points
Part #:
6FTAUPEH
List Price
USD 14,380.00
HTRF Tau Aggregation Kit, 10,000 Assay Points
HTRF Tau Aggregation aggregation Detection Kit
HTRF Tau Aggregation Kit, 10,000 Assay Points
Product information
Overview
Tau proteins are required to stabilize microtubules through tubulin association. Deregulation of Tau expression, aggregation, and subsequent phosphorylation have been correlated to the onset of pathologies linked to the nervous system such as Alzheimer's and Parkinson's. The Tau aggregation assay detects human Tau aggregates rapidly in brain tissues and cell cultures. This kit is part of our Tau range, including phosphorylated Tau detection and total Tau measurement (MAP-Tau).
Specifications
| Application |
Protein Quantification
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Kit
|
| Sample Volume |
10 µL
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target |
Kit
|
| Target Class |
Biomarkers
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Neuroscience
|
| Unit Size |
10,000 Assay Points
|
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HTRF Tau Aggregation Kit, 10,000 Assay Points
HTRF Tau Aggregation Kit, 10,000 Assay Points
How it works
Assay principle
Tau aggregates are measured using a sandwich immunoassay involving an anti-Tau monoclonal antibody specific for Tau aggregate labelled with terbium-Cryptate or d2, ensuring assay quality, reproducibility, and signal quality. The specific HTRF signal generated is proportional to the Tau aggregates.
Assay Protocol
The sample or brain extracts are transferred to the assay plate for the detection of Tau aggregates. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.
Assay details
Assay specificity & linearity
A FRET signal is generated in the presence of aggregated tau, leading to a distinctive signal compared to non-aggregated tau. The assay shows a good linear correlation between signal intensity and the purified recombinant tau aggregate concentrations obtained by serial dilutions in assay diluent, with a linear range of 1.9 to 500 ng/mL.
Assay validation
Tau aggregation kinetic study
Recombinant full-length human tau protein was chemically aggregated. The reaction was stopped at different times (1, 2, 4, 6 and 24 h). The samples were previously diluted (1/500) to follow the kinetics of aggregation. This assay could also be used to monitor a kinetic of dissociation after a compound addition. The test was performed in 384-well low volume plates, read on a PHERAstar FS laser.
Resources
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Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
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