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HTRF Tag-Lite β2 Adrenergic (HEK293) Receptor Cell line

Diagram of Tag-lite Adrenergic beta2 stably expressing cells image

This cell line stably expresses the Adrenergic beta2 receptor fused to a SNAP-Tag, and can be used in a Tag-lite application.

For research use only. Not for use in diagnostic procedures.

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Feature	Specification
Application	Receptor-Ligand Binding

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This cell line stably expresses the Adrenergic beta2 receptor fused to a SNAP-Tag, and can be used in a Tag-lite application.

For research use only. Not for use in diagnostic procedures.

View product information

Product Variant

Unit Size: 1 Item

Part #:

C1SU1BETA2

List Price

USD 14,900.00

Product information

Overview

Endothelin receptors can be divided into ETA and ETB receptors, also called ETA1 and ETA2. Both receptors are involved in the treatment of hypertension.

This HEK293 stable cell line expresses the BETA2 receptor fused to a SNAP-Tag. Labeled with Terbium, the cells can be used in a Tag-lite receptor binding protocol.

Specifications

Other Specifications

Application	Receptor-Ligand Binding
Brand	Tag-lite
Detection Modality	HTRF
Product Group	Cells
Shipping Conditions	Shipped in Dry Ice
Target Class	GPCR
Technology	TR-FRET
Therapeutic Area	Cardiovascular Infectious Diseases Metabolism/Diabetes NASH/Fibrosis Neuroscience Oncology & Inflammation Rare Diseases
Unit Size	1 Item

Video gallery

How it works

Step 1 - Receptor labeling

SNAP-tag®, is a small fusion tag that covalently interacts with specific substrates. SNAP-tag allows specific and covalent labeling of any protein of interest. For more details, see the labeling procedure.

Step 2 - Understand the assay principle

Running a receptor binding assay using Tag-lite is as easy as it can get. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.

Step 3 - Saturation binding (KD)

A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.

how-it-works-adrenergic-beta2-cell-line-hek293

Step 4 - Competitive binding (KI)

A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.

Assay validation

Kd and Ki validation

Examples of data obtained using adrenergic beta 2-R expressing stable cells (HEK) labelled with terbium in Tag-lite buffer (LABMED), and their matching fluorescent ligand (L0011GRE). ICI-118551 was used as reference ligand. Results may vary from one HTRF® compatible reader to another.