Tag-lite cells transiently expressing the Serotonin 5HT4 receptor labeled with Terbium for use in receptor binding applications. The cells are provided ready to use.
For research use only. Not for use in diagnostic procedures.
The 5-HT4 receptor is a postsynaptically located GPCR present at highest densities in the brain. The 5-HT4 GPCR plays a role in learning and memory.
Cells expressing the 5HT4 receptor are provided pre-labeled with Terbium, and can be used to conduct receptor binding studies on the aforementioned receptor.
Assay Points |
200
|
---|---|
Assay Target Type |
Cells
|
Assay Technology |
HTRF
|
Brand |
Tag-Lite
|
Quantity |
1
|
Shelf Life |
540.0 Day(s)
|
Shipping Conditions |
Shipped in Dry Ice
|
Therapeutic Area |
Cardiovascular
Infectious Diseases
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
Rare Diseases
|
Unit Size |
200 Assay Points
|
Running your 5HT4 receptor binding assay using Tag-lite is as easy as it can gets. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.
A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.
A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.
Examples of data obtained using Serotonin 5HT4 labeled cells and their matching fluorescent ligand (L0043RED). GR-113808 was used as reference ligand. Results may vary from one HTRF® compatible reader to another.
Tag-lite™ is a cell-based, no-wash alternative to traditional radioisotope assays for studying GPCR-ligand interaction.
Adenosine...
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