SNAP-Tag, Clip-Tag, and HALO-Tag are protein labeling systems enabling the covalent attachement of fluorophores onto proteins of interest. SNAP-Lumi4-Tb enables SNAP-tagged proteins to become labeled with a Terbium fluorophore.
For research use only. Not for use in diagnostic procedures.
Over the past few years, SNAP-Tag, CLIP-Tag, and Halo-Tag technologies combined with TR-FRET have paved the road to the development of many non-radioactive, no-wash binding assays. The method is based on transfecting cells using plasmids encoding a SNAP-Tag (or CLIP-Tag, or Halot-Tag) and subsequently labeling them with acceptors or donors. SNAP-Lumi4-Tb allows the labeling of SNAP-Tagged proteins with a terbium fluorophore.
Assay Points |
25
|
---|---|
Assay Target Type |
Fluorescent reagent
|
Assay Technology |
HTRF
|
Brand |
Tag-Lite
|
Quantity |
1
|
Shipping Conditions |
Shipped in Dry Ice
|
Therapeutic Area |
Cardiovascular
Infectious Diseases
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
Rare Diseases
|
Unit Size |
25 nmol
|
SNAP-tag® is a small fusion tag that covalently interacts with specific substrates. SNAP-tag enables the specific and covalent labeling of any protein of interest. Cells are provided unlabeled and need to be labeled with Lumi4-Terbium prior to running a binding assay. Labeling reagents are available from the Revvity catalog in 4 different sizes. For more details refer to labeling procedure.
This guide provides you an overview of HTRF applications in several therapeutic areas.
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