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HTRF Tag-Lite pSNAP-Adenosine A1 Receptor Plasmid

Image of the vial of pSNAP-Adenosine receptor A1 image

The Tag-lite A1 Adenosine receptor plasmid is used to transiently or stably transfect cells in order to develop an A1 Adenosine receptor binding assay.

For research use only. Not for use in diagnostic procedures.

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Feature	Specification
Application	Receptor-Ligand Binding

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The Tag-lite A1 Adenosine receptor plasmid is used to transiently or stably transfect cells in order to develop an A1 Adenosine receptor binding assay.

For research use only. Not for use in diagnostic procedures.

View product information

Product Variant

Unit Size: 1 Unit

Part #:

PSNAPA1

List Price

USD 1,923.00

Product information

Overview

Over past few years, SNAP-Tag technology combined with TR-FRET has paved way development of many non-radioactive, no-wash, binding assays. method is based on transfecting cells using plasmids encoding a SNAP-Tag, and subsequently labeling m with Terbium. Revvity offers a large collection of such plasmids. All GPCR genes are cloned in an expression vector directly downstream from a CMV promoter, and are provided ready protein expression and labeling.

All information on this page pertains to Tag-lite plasmid cloned with Adenosine A1 receptor.

Specifications

Other Specifications

Application	Receptor-Ligand Binding
Brand	Tag-lite
Detection Modality	HTRF
Product Group	Plasmids
Shipping Conditions	Shipped in Dry Ice
Target Class	GPCR
Technology	TR-FRET
Therapeutic Area	Cardiovascular Infectious Diseases Inflammation Metabolism/Diabetes NASH/Fibrosis Neuroscience Oncology & Inflammation Rare Diseases
Unit Size	1 Unit

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How it works

Step 1 - Plasmid transfection

Use standard transfection techniques (refer to transient transfection protocol) to transiently express the SNAP-GPCR of interest in your cell line.

Step 2 - Receptor labeling

SNAP-tag® is a small fusion tag that covalently interacts with specific substrates. It enables the specific and covalent labeling of any protein of interest (refer to labeling procedure). Cells are provided unlabeled, and need to be labeled with Lumi4-Terbium prior to running a binding assay. Labeling reagents are available in 4 different sizes from the Revvity catalog.

Step 3 - Understand the assay principle

Running a receptor binding assay using Tag-lite is as easy as it can get. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.

Step 4- Saturation binding (KD)

A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells, and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.

how-it-works-psnap-adenosine-a1-receptor

Step 5 - Competitive binding (KI)

A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.