HTRF PolyGT-Biotin substrate, 5,000 Assay Points

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HTRF PolyGT-Biotin substrate, 5,000 Assay Points

SDS

COAs

TDS

IFUs, Manuals & more

PolyGT-Biotin has been shown to be a substrate for tyrosine-specific protein kinases.

Product information

For research use only. Not for use in diagnostic procedures.

SDS

COAs

TDS

IFUs, Manuals & more

Product information

Overview

Poly-(Glu,Tyr) (4:1) is a random copolymer which has been shown to be a substrate for tyrosine-specific protein kinases. Poly-(GT)-biotin may be used with Streptavidin-XL665 and Anti-phosphotyrosine-cryptate for easily optimized HTRF® tyrosine kinase assays. HTRF enables to perform assay at physiological ATP concentrations.

Specifications

Other Specifications

Assay Points	5000
Assay Technology	HTRF
Brand	HTRF
Product Group	Other Reagent
Quantity	1
Shipping Conditions	Shipped Ambient
Therapeutic Area	Cardiovascular Infectious Diseases Inflammation Metabolism/Diabetes NASH/Fibrosis Neuroscience Oncology & Inflammation Rare Diseases
Unit Size	5,000 Assay Points

Video gallery

How it works

Assay principles

HTRF Tyrosine kinase assay use typically a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and PolyGT-Biotin as substrate. The signal is proportional to the concentration of phospho-tyrosine.

Assay protocol

HTRF tyrosine kinase assay have two basic phases: 1. Enzymatic step: biotinylated PolyGT is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated PolyGT is then detected by the addition of Streptavidin-XL665 and a Europium-cryptate labeled anti-phosphotyrosine antibody (MAb PY20-Eu cryptate or MAb PT66-Eu cryptate) prepared in a buffer containing EDTA to stop the enzymatic reaction.