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HTRF Phospho-Total protein lysis buffer 1, 130 ml
Lysis buffer#1 is one of the components for protein extraction provided with HTRF total and phospho kits (cf. lysis buffer compatiblity table). It can be ordered as a separate consumable.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product information
Overview
Correct buffer conditions are essential for optimal cell lysis and extraction of proteins. The composition of the phospho / total protein lysis buffer 1 has been carefully optimized for the quantification of protein expression and phosphorylation. You may refer to the lysis buffer compatibility table to find out which assays work best with buffer 1.
Specifications
| Assay Points |
130
|
|---|---|
| Assay Target Type |
Buffer
|
| Assay Technology |
HTRF
|
| Brand |
HTRF
|
| Quantity |
1
|
| Unit Size |
130 ml
|
Video gallery
Citations
Assay details
Lysis buffer compatibility table
Revvity's portfolio of phospho and total protein assays has been optimized with 4 different lysis buffers. In most instances, one, sometimes two buffers among the four work significantly better for a given assay. You may refer the compatibility table to find out which buffer you should use preferentially with your assay of interest. This table will also allow you to pick a buffer working equally well with several assays, in order to run them using the same lysate.
Resources
Compound MoA and potential cytotoxic effects deciphered thanks to immunoassays and cell viability assay
The attrition of drug...
Increase the flexibility of your phospho-protein assays
This Application Note compares RIPA and HTRF™ phospho-total lysis buffer in...
This guide provides you an overview of HTRF applications in several therapeutic areas.
This application note demonstrates how compound’s mechanism of action and potential cytotoxic effects can be deciphered thanks to...
SDS, COAs, Manuals and more
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