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HTRF Phospho and Total protein blocking reagent, 2 ml

This reagent is a component of total and phospho- protein kits and prevents protein degradation after cell lysis.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature Specification
Application Cell Signaling

This reagent is a component of total and phospho- protein kits and prevents protein degradation after cell lysis.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 2 ml
Part #:
64KB1AAC
List Price
USD 622.06
Unit Size: 6 ml
Part #:
64KB1AAD
List Price
USD 1,219.00

Overview

Proteins and phosphoprotein may be prone to degradation following cell lysis, e.g. by endogeneous phosphatases. When running a cell-based assay, an appropriate stabilizing and blocking reagent is thus mandatory in order to prevent any remaining enzymatic activity, as well as to garantee protein integrity and quantification.

Specifications

Application
Cell Signaling
Brand
HTRF
Detection Modality
HTRF
Product Group
Buffer
Shipping Conditions
Shipped in Dry Ice
Target Class
Phosphoproteins
Technology
TR-FRET
Unit Size
2 ml

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Resources

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Application Note
HTRF phospho-total lysis buffer: a universal alternative to RIPA lysis buffers

Increase the flexibility of your phospho-protein assays

This Application Note compares RIPA and HTRF™ phospho-total lysis buffer in...

Application Note Icon
Application Note
Simultaneous detection of drug efficacy and toxicity by combining HTRF, AlphaLISA, or AlphaLISA SureFire Ultra with ATPlite

This application note demonstrates how compound’s mechanism of action and potential cytotoxic effects can be deciphered thanks to...

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