US
Search Results
Revvity Sites Globally
Select your location.
*e-commerce not available for this region.
HTRF Mouse TNFα Detection Kit, 500 Assay Points
The HTRF mouse TNF alpha kit is designed for the quantification of mouse TNF alpha release in cell supernatant.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Product information
Overview
TNF alpha is a chemokine mainly produced by macrophages, T cells, and neutrophils. TNF alpha acts as a chemoattractant for neutrophils, and promotes infiltration and activation at inflammation sites. TNF alpha is involved in several cancer types for its ability to promote angiogenesis and cell proliferation, as well as to inhibit apoptosis.
Specifications
| Assay Points |
500
|
|---|---|
| Assay Target Type |
Kit
|
| Assay Technology |
HTRF
|
| Brand |
HTRF
|
| Quantity |
1
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Therapeutic Area |
Metabolism/Diabetes
NASH/Fibrosis
Oncology & Inflammation
|
| Unit Size |
500 Assay Points
|
Video gallery
Citations
How it works
Assay principle
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
Assay data analysis
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.
Revvity also worked with Myassays.com to help you in your data analysis.
Assay details
Technical specifications of mouse TNF alpha kit
| Sample size | 16 µL |
|---|---|
| Final assay volume | 20 µL |
| Kit components | Lyophilized standard, frozen detection antibodies, buffers &protocol. |
| LOD &LOQ (in Diluent) | 3 pg/mL &20 pg/mL |
| Range | 20 6,000 pg/mL |
| Time to result | 1h at RT |
| Species | Mouse only |
Analytical performance
Intra and inter assay
Intra-assay (n=24)
| Sample | Mean [TNFa] (pg/mL) | CV |
|---|---|---|
| 1 | 57 |
7% |
| 2 | 2570 | 2% |
| 3 | 5256 |
2% |
| Mean CV |
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
Inter-assay (n=4)
| Sample | [TNFa] (pg/mL) | Mean (delta R) | CV |
|---|---|---|---|
| 1 | 93 | 457 | 2% |
| 2 | 493 | 2182 | 3% |
| 3 | 2609 | 8729 | 3% |
| Mean CV | 3% |
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
Resources
Guide
Cytokine assays: a guide to success with HTRF
The definitive guide to setting up a successful cytokine assay
Many therapeutic areas require an understanding of cytokine release...
Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
SDS, COAs, Manuals and more
Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.
How can we help you?
We are here to answer your questions.
